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A chromatic explosion: the development and future of multiparameter flow cytometry
Summary Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry a...
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Published in: | Immunology 2008-12, Vol.125 (4), p.441-449 |
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Language: | English |
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cites | cdi_FETCH-LOGICAL-c5039-6c6e8c1a7470d25f55d320438d5c81f8e55efa9e0aa35750ec854c541a51aa4f3 |
container_end_page | 449 |
container_issue | 4 |
container_start_page | 441 |
container_title | Immunology |
container_volume | 125 |
creator | Chattopadhyay, Pratip K. Hogerkorp, Carl‐Magnus Roederer, Mario |
description | Summary
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity. |
doi_str_mv | 10.1111/j.1365-2567.2008.02989.x |
format | article |
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Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1111/j.1365-2567.2008.02989.x</identifier><identifier>PMID: 19137647</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Coloring Agents ; data analysis ; Equipment Design ; flow cytometry ; Flow Cytometry - methods ; Flow Cytometry - trends ; fluorochromes ; Forecasting ; Humans ; Immune System Diseases - diagnosis ; Review ; Statistics as Topic ; T cells ; tetramers</subject><ispartof>Immunology, 2008-12, Vol.125 (4), p.441-449</ispartof><rights>2008 Blackwell Publishing Ltd. No claim to original US government works</rights><rights>2008 Blackwell Publishing Ltd 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5039-6c6e8c1a7470d25f55d320438d5c81f8e55efa9e0aa35750ec854c541a51aa4f3</citedby><cites>FETCH-LOGICAL-c5039-6c6e8c1a7470d25f55d320438d5c81f8e55efa9e0aa35750ec854c541a51aa4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612557/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612557/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19137647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chattopadhyay, Pratip K.</creatorcontrib><creatorcontrib>Hogerkorp, Carl‐Magnus</creatorcontrib><creatorcontrib>Roederer, Mario</creatorcontrib><title>A chromatic explosion: the development and future of multiparameter flow cytometry</title><title>Immunology</title><addtitle>Immunology</addtitle><description>Summary
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.</description><subject>Animals</subject><subject>Coloring Agents</subject><subject>data analysis</subject><subject>Equipment Design</subject><subject>flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Flow Cytometry - trends</subject><subject>fluorochromes</subject><subject>Forecasting</subject><subject>Humans</subject><subject>Immune System Diseases - diagnosis</subject><subject>Review</subject><subject>Statistics as Topic</subject><subject>T cells</subject><subject>tetramers</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhi0EokvhLyCfuCX4I5PYSCBVVYFKrZAQnC3jjNmsnDjYSbv770nYVYET-GKP5plXYz2EUM5KvpzXu5LLGgoBdVMKxlTJhFa63D8im4fGY7JhjOtCKAZn5FnOu6WUDOApOeOay6aumg35fEHdNsXeTp2juB9DzF0c3tBpi7TFOwxx7HGYqB1a6udpTkijp_0cpm60yfY4YaI-xHvqDlNcynR4Tp54GzK-ON3n5Ov7qy-XH4ubTx-uLy9uCgdM6qJ2NSrHbVM1rBXgAVopWCVVC05xrxAAvdXIrJXQAEOnoHJQcQvc2srLc_LumDvO33ps3bJmssGMqettOphoO_N3Z-i25nu8M6LmAqBZAl6dAlL8MWOeTN9lhyHYAeOcTa211CCqf4JcA2hgbAHVEXQp5pzQP2zDmVnNmZ1ZBZlVkFnNmV_mzH4Zffnnb34PnlQtwNsjcN8FPPx3sLm-vV1f8idip6mZ</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Chattopadhyay, Pratip K.</creator><creator>Hogerkorp, Carl‐Magnus</creator><creator>Roederer, Mario</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200812</creationdate><title>A chromatic explosion: the development and future of multiparameter flow cytometry</title><author>Chattopadhyay, Pratip K. ; Hogerkorp, Carl‐Magnus ; Roederer, Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5039-6c6e8c1a7470d25f55d320438d5c81f8e55efa9e0aa35750ec854c541a51aa4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Coloring Agents</topic><topic>data analysis</topic><topic>Equipment Design</topic><topic>flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>Flow Cytometry - trends</topic><topic>fluorochromes</topic><topic>Forecasting</topic><topic>Humans</topic><topic>Immune System Diseases - diagnosis</topic><topic>Review</topic><topic>Statistics as Topic</topic><topic>T cells</topic><topic>tetramers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chattopadhyay, Pratip K.</creatorcontrib><creatorcontrib>Hogerkorp, Carl‐Magnus</creatorcontrib><creatorcontrib>Roederer, Mario</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chattopadhyay, Pratip K.</au><au>Hogerkorp, Carl‐Magnus</au><au>Roederer, Mario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A chromatic explosion: the development and future of multiparameter flow cytometry</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>2008-12</date><risdate>2008</risdate><volume>125</volume><issue>4</issue><spage>441</spage><epage>449</epage><pages>441-449</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><abstract>Summary
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19137647</pmid><doi>10.1111/j.1365-2567.2008.02989.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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source | Wiley-Blackwell Read & Publish Collection; PubMed Central |
subjects | Animals Coloring Agents data analysis Equipment Design flow cytometry Flow Cytometry - methods Flow Cytometry - trends fluorochromes Forecasting Humans Immune System Diseases - diagnosis Review Statistics as Topic T cells tetramers |
title | A chromatic explosion: the development and future of multiparameter flow cytometry |
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