Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy

A factor that limits the use of multiphoton microscopy (MPM) in clinical and preclinical studies is the lack of a compact and flexible probe. We report on a miniaturized MPM probe employing a microelectromechanical system (MEMS) scanning mirror and a double-clad photonic crystal fiber (DCPCF). The u...

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Bibliographic Details
Published in:Optics letters 2008-06, Vol.33 (12), p.1324-1326
Main Authors: JUNG, Woonggyu, SUO TANG, MCCORMIC, Daniel T, TIQUIANG XIE, AHN, Yeh-Chan, JIANPING SU, TOMOV, Ivan V, KRASIEVA, Tatiana B, TROMBERG, Bruce J, ZHONGPING CHEN
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Nucleus - ultrastructure
Chondrocytes - ultrastructure
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Fundamental areas of phenomenology (including applications)
General aspects, investigation technics, apparatus
Humans
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Nonlinear optics
Optics
Physics
Tissues, organs and organisms biophysics
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Summary:A factor that limits the use of multiphoton microscopy (MPM) in clinical and preclinical studies is the lack of a compact and flexible probe. We report on a miniaturized MPM probe employing a microelectromechanical system (MEMS) scanning mirror and a double-clad photonic crystal fiber (DCPCF). The use of a MEMS mirror and a DCPCF provides many advantages, such as size reduction, rapid and precise scanning, efficient delivery of short pulses, and high collection efficiency of fluorescent signals. The completed probe was 1 cm in outer diameter and 14 cm in length. The developed probe was integrated into an MPM system and used to image fluorescent beads, paper, and biological specimens.
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.33.001324