Initiation of New DNA Strands by the Herpes Simplex Virus-1 Primase-Helicase Complex and Either Herpes DNA Polymerase or Human DNA Polymerase α

A key set of reactions for the initiation of new DNA strands during herpes simplex virus-1 replication consists of the primase-catalyzed synthesis of short RNA primers followed by polymerase-catalyzed DNA synthesis (i.e. primase-coupled polymerase activity). Herpes primase (UL5-UL52-UL8) synthesizes...

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Bibliographic Details
Published in:The Journal of biological chemistry 2009-01, Vol.284 (3), p.1523-1532
Main Authors: Cavanaugh, Nisha A., Kuchta, Robert D.
Format: Article
Language:English
Subjects:
DNA Helicases - metabolism
DNA Polymerase I - metabolism
DNA Primase - metabolism
DNA Primers - metabolism
DNA, Viral - biosynthesis
DNA-Directed DNA Polymerase - metabolism
DNA: , Repair, Recombination, and Chromosome Dynamics
Exodeoxyribonucleases - metabolism
Herpesvirus 1, Human - physiology
Humans
RNA - metabolism
Viral Proteins - metabolism
Virus Replication - physiology
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Summary:A key set of reactions for the initiation of new DNA strands during herpes simplex virus-1 replication consists of the primase-catalyzed synthesis of short RNA primers followed by polymerase-catalyzed DNA synthesis (i.e. primase-coupled polymerase activity). Herpes primase (UL5-UL52-UL8) synthesizes products from 2 to ∼13 nucleotides long. However, the herpes polymerase (UL30 or UL30-UL42) only elongates those at least 8 nucleotides long. Surprisingly, coupled activity was remarkably inefficient, even considering only those primers at least 8 nucleotides long, and herpes polymerase typically elongated
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M805476200