Extraction of root-associated Meloidogyne incognita and Rotylenchulus reniformis

A technique based on physical maceration of root tissue was developed to extract vermiform and swollen stages of Meloidogyne incognita and Rotylenchulus reniformis. Experiments conducted on soybean and tomato evaluated the efficiency of method (stir, grind), NaOCl concentration (0%, 0.5%), and durat...

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Bibliographic Details
Published in:Journal of nematology 1997-06, Vol.29 (2), p.209-215
Main Authors: Stetina, S.R, McGawley, E.C, Russin, J.S
Format: Article
Language:English
Subjects:
CHLORE
CHLORINE
CLORO
DESINFECTANT
DESINFECTANTES
DISINFECTANTS
EXTRACCION
EXTRACTION
GLYCINE MAX
LYCOPERSICON ESCULENTUM
MACERACION
MACERATING
MACERATION
MELOIDOGYNE INCOGNITA
METHODE
METHODOLOGY
METHODS
METODOS
NEMATODE DES PLANTES
NEMATODE EXTRACTION
NEMATODOS DE LAS PLANTAS
OVA
OVULE
OVULO
PLANT NEMATODES
RACINE
RAICES
RENIFORM NEMATODES
ROOT KNOT NEMATODES
ROOTS
ROTYLENCHULUS RENIFORMIS
SODIUM HYPOCHLORITE
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Summary:A technique based on physical maceration of root tissue was developed to extract vermiform and swollen stages of Meloidogyne incognita and Rotylenchulus reniformis. Experiments conducted on soybean and tomato evaluated the efficiency of method (stir, grind), NaOCl concentration (0%, 0.5%), and duration (1x, 2x) on extraction of nematodes and eggs from 60-day-old populations. Root-associated populations of R. reniformis were considerably lower than those of M. incognita, so development of the method focused on the latter. Grinding liberated more nematodes than stirring, but the reverse was true for egg extraction. Among grinding treatments, a duration of 10 seconds in 0.5% NaOCl provided the most efficient extraction of nematodes and eggs. Among stirring treatments, a duration of 10 minutes in 0.5% NaOCl provided the most efficient extraction of eggs. These techniques were compared on soybean roots 30 days older than those on which the procedures were first evaluated, with consistent results
ISSN:0022-300X
2640-396X