Heterodera glycines cyst components and surface disinfestants affect H. glycines hatching

We investigated the effects of Heterodera glycines cyst components and surface disinfestants on hatching of H. glycines eggs in vitro. Eggs were incubated in either H. glycines cyst wall fragments, cyst wall and egg rinsate, egg homogenate, or control solutions of soybean root diffusate, sterile dis...

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Bibliographic Details
Published in:Journal of nematology 2003-12, Vol.35 (4), p.458-464
Main Authors: Charlson, D.V, Tylka, G.L
Format: Article
Language:English
Subjects:
antimicrobial agents
cyst nematodes
cysts (developmental stages)
disinfestation
Ecology and Biology
egg hatchability
Glycine max
hatch inhibition
hatch stimulation
incubation
incubation media
inhibitors
soybeans
surface disinfestation
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Summary:We investigated the effects of Heterodera glycines cyst components and surface disinfestants on hatching of H. glycines eggs in vitro. Eggs were incubated in either H. glycines cyst wall fragments, cyst wall and egg rinsate, egg homogenate, or control solutions of soybean root diffusate, sterile distilled water, or zinc sulfate. Hatch in cyst wall and egg rinsate, and egg homogenate, was greater (alpha = 0.05) than hatch in sterile distilled water; however, it was not different from hatch in zinc sulfate according to Dunnett's test. Hatch in cyst wall fragments was similar to hatch in sterile distilled water. To determine whether surface disinfestants affected hatch, eggs were treated first with chlorhexidine diacetate, mercuric chloride, sodium hypochlorite, or streptomycin sulfate and then incubated in H. glycines egg homogenate, soybean root diffusate, sterile distilled water, or zinc sulfate. Hatch of eggs treated with chlorhexidine diacetate, mercuric chloride, and streptomycin sulfate was reduced (alpha = 0.05), and hatch of eggs treated with sodium hypochlorite was increased (alpha = 0.05) relative to hatch of nontreated eggs in all incubation solutions except zinc sulfate according to Dunnett's Test. Hatch in zinc sulfate was similar among all surface disinfestants except mercuric chloride, where hatch was reduced relative to hatch of nontreated and other surface disinfestant-treated eggs.
ISSN:0022-300X
2640-396X