Purification and preliminary crystallographic analysis of a new Lys49-PLA2 from B. Jararacussu

BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the...

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Bibliographic Details
Published in:International journal of molecular sciences 2008-05, Vol.9 (5), p.736-750
Main Authors: dos Santos, Marcelo L, Fagundes, Fábio H R, Teixeira, Bruno R F, Toyama, Marcos H, Aparicio, Ricardo
Format: Article
Language:English
Subjects:
Blood platelets
Chromatography
Crystallization
Crystals
Kinases
Physiology
Proteins
Snakes
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Summary:BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 A resolution and 1.9 A resolution for crystals belonging to the space group P2(1)2(1)2(1) (a = 48.4 A, b = 65.3 A, c = 84.3 A) and space group P3(1)21 (a = b = 55.7 A, c = 127.9 A), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms9050736