Oxygen Microscopy by Two-Photon-Excited Phosphorescence

High‐resolution images of oxygen distributions in microheterogeneous samples are obtained by two‐photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two‐photon absorption (2PA) cross‐section. In this probe, energy is harvested by a 2PA ante...

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Bibliographic Details
Published in:Chemphyschem 2008-08, Vol.9 (12), p.1673-1679
Main Authors: Finikova, Olga S., Lebedev, Artem Y., Aprelev, Alexey, Troxler, Thomas, Gao, Feng, Garnacho, Carmen, Muro, Silvia, Hochstrasser, Robin M., Vinogradov, Sergei A.
Format: Article
Language:English
Subjects:
Atomic and molecular collision processes and interactions
Atomic and molecular physics
Biological and medical sciences
Cells, Cultured
dendrimer
Endothelial Cells
energy transfer
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Fundamental areas of phenomenology (including applications)
General aspects, investigation technics, apparatus
Humans
Intramolecular energy transfer
intramolecular dynamics
dynamics of van der waals molecules
Luminescent Measurements
Microscopy - methods
Molecular Structure
Nanostructures
Nonlinear optics
Optical susceptibility, hyperpolarizability
Optics
oxygen
Oxygen - chemistry
phosphorescence
Photochemistry
Photons
Physics
porphyrin
Tissues, organs and organisms biophysics
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Summary:High‐resolution images of oxygen distributions in microheterogeneous samples are obtained by two‐photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two‐photon absorption (2PA) cross‐section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction‐limited resolution, and lifetime‐based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO2 in cells. Oxygen distributions are imaged by two‐photon laser scanning microscopy (2P LSM) using a newly developed two‐photon‐enhanced phosphorescent nanoprobe (see figure). 2P LSM allows visualization of oxygen gradients in 3D with near diffraction‐limited resolution, and lifetime‐based measurements eliminate dependence on the local probe concentration.
ISSN:1439-4235
1439-7641
DOI:10.1002/cphc.200800296