Novel Polyamine Acyltransferase Responsible for the Accumulation of Spermidine Conjugates in Arabidopsis Seed

Hydroxycinnamic acid amides are a class of secondary metabolites distributed widely in plants. We have identified two sinapoyl spermidine derivatives, N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N,N'-disinapoylspermidine, which comprise the two major polyamine conjugates th...

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Bibliographic Details
Published in:The Plant cell 2009-01, Vol.21 (1), p.318-333
Main Authors: Luo, Jie, Fuell, Christine, Parr, Adrian, Hill, Lionel, Bailey, Paul, Elliott, Katherine, Fairhurst, Shirley A, Martin, Cathie, Michael, Anthony J
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Acyltransferases - metabolism
Amides
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Coumaric acids
DNA, Bacterial - genetics
Enzymes
Gene Expression Regulation, Plant
Gene Knockout Techniques
Genes
Genetic Complementation Test
Germination
Metabolites
Metabolome
Mutagenesis, Insertional
Plant biochemistry
Plant roots
Plants
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Polyamines
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
RNA, Plant - genetics
Secondary metabolites
Seedlings
Seeds - genetics
Seeds - metabolism
Spermidine - biosynthesis
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Summary:Hydroxycinnamic acid amides are a class of secondary metabolites distributed widely in plants. We have identified two sinapoyl spermidine derivatives, N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N,N'-disinapoylspermidine, which comprise the two major polyamine conjugates that accumulate in Arabidopsis thaliana seed. Using metabolic profiling of knockout mutants to elucidate the functions of members of the BAHD acyltransferase family in Arabidopsis, we have also identified two genes encoding spermidine disinapoyl transferase (SDT) and spermidine dicoumaroyl transferase (SCT) activities. At2g23510, which is expressed mainly in seeds, encodes a spermidine sinapoyl CoA acyltransferase (SDT) that is required for the production of disinapoyl spermidine and its glucoside in Arabidopsis seed. The structurally related BAHD enzyme encoded by At2g25150 is expressed specifically in roots and has spermidine coumaroyl CoA acyltransferase (SCT) activity both in vitro and in vivo.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.108.063511