SHED repair critical-size calvarial defects in mice

Objective:  Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo‐inductive cells. To examine whether SHED‐mediated bone regeneration can be utilized for...

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Published in:Oral diseases 2008-07, Vol.14 (5), p.428-434
Main Authors: Seo, BM, Sonoyama, W, Yamaza, T, Coppe, C, Kikuiri, T, Akiyama, K, Lee, JS, Shi, S
Format: Article
Language:English
Subjects:
Absorbable Implants
Animals
Biocompatible Materials
bone
Bone Cements
Bone Regeneration - physiology
Bone Substitutes
Calcium Phosphates
Cells, Cultured
Dentistry
Durapatite
Humans
Mice
Mice, Inbred Strains
Multipotent Stem Cells - cytology
Multipotent Stem Cells - transplantation
Neural Crest - cytology
Osseointegration - physiology
osteoblast
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis - physiology
regeneration
Skull - surgery
Stem Cell Transplantation - methods
stem cells from human exfoliated deciduous teeth (SHED)
Tooth, Deciduous - cytology
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Summary:Objective:  Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo‐inductive cells. To examine whether SHED‐mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical‐size calvarial defects in immunocompromised mice. Materials and methods:  We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas. Results:  SHED were able to repair the defects with substantial bone formation. Interestingly, SHED‐mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell‐generated bone. Furthermore, SHED were found to co‐express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential. Conclusions:  Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.
ISSN:1354-523X
1601-0825
DOI:10.1111/j.1601-0825.2007.01396.x