Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study)

• Published in: World journal of gastroenterology : WJG 2009-01, Vol.15 (1), p.112-116
• Main Authors: Adas, Gokhan, Arikan, Soykan, Kemik, Ozgur, Oner, Ali, Sahip, Nilgun, Karatepe, Oguzhan
• Format: Article
• Language: English
• Subjects: Albendazole - administration & dosage; Albendazole - analogs & derivatives; Animals; Anthelmintics - administration & dosage; Brief; Echinococcosis, Hepatic - drug therapy; Echinococcosis, Hepatic - parasitology; Echinococcus granulosus - drug effects; Echinococcus granulosus - isolation & purification; Humans; In Vitro Techniques; Solutions
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.112

To establish which scolicidal agents are superior and more suitable for regular use. Echinococcus granulosus protoscoleces were obtained from 25 patients with liver hydatid cysts. Various concentrations of albendazole sulfone, albendazole sulfoxide, and albendazole sulfone and albendazole sulfoxide mixed together in concentrations of 50 microg/mL, and H(2)O(2) in a concentration of 4%, NaCl 20%, and 1.5% cetrimide-0.15% chlorhexidine (10% Savlon-Turkey) were used to determine the scolicidal effects. Albendazole (ABZ) derivatives and other scolicidal agents were applied to a minimum of 100 scoleces for 5 and 10 min. The degree of viability was calculated according to the number of living scolices per field from a total of 100 scolices observed under the microscope. After 5 min, ABZ sulfone was 97.3% effective, ABZ sulfoxide was 98.4% effective, and the combined solution was 98.6% effective. When sulfone, sulfoxide and the combined solutions were compared, the combined solution seemed more effective than sulfone. However, there was no difference when the combined solution was compared with sulfoxide. After 10 min, hypertonic salt water, sulfone, sulfoxide, and the combined solution compared to other solutions looked more effective and this was statistically significant on an advanced level. When sulfone, sulfoxide, and the combined solutions were compared with each other, the combined solution appeared more effective than sulfone. When the combined solution was compared with sulfoxide, there was no difference. Despite being active, ABZ metabolites did not provide a marked advantage over 20% hypertonic saline. According to these results, we think creating a newly improved and more active preparation is necessary for hydatid cyst treatment.