Transgenic mice with defined combinations of drug-inducible reprogramming factors

Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary'...

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Bibliographic Details
Published in:Nature biotechnology 2009-02, Vol.27 (2), p.169-171
Main Authors: Jaenisch, Rudolf, Markoulaki, Styliani, Hanna, Jacob, Beard, Caroline, Carey, Bryce W, Cheng, Albert W, Lengner, Christopher J, Dausman, Jessica A, Fu, Dongdong, Gao, Qing, Wu, Su, Cassady, John P
Format: Article
Language:English
Subjects:
Agriculture
Animals
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
brief-communication
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cells, Cultured
Chimera - genetics
Chimera - metabolism
DNA binding proteins
Doxycycline - pharmacology
Female
Fibroblasts - metabolism
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetic Techniques
Genetically modified mice
Genetics
Health aspects
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Kruppel-Like Transcription Factors - genetics
Kruppel-Like Transcription Factors - metabolism
Life Sciences
Male
Mice
Mice, Transgenic
Miscellaneous
Octamer Transcription Factor-3 - genetics
Octamer Transcription Factor-3 - metabolism
Physiological aspects
Pluripotent Stem Cells
Proto-Oncogene Proteins c-myc - genetics
Proto-Oncogene Proteins c-myc - metabolism
Proviruses - genetics
Proviruses - metabolism
Rodents
SOXB1 Transcription Factors - genetics
SOXB1 Transcription Factors - metabolism
Stem cells
Transcription Factors - drug effects
Transcription Factors - genetics
Transgenic animals
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Description
Summary:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt.1520