Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats

• Published in: World journal of gastroenterology : WJG 2009-03, Vol.15 (11), p.1339-1345
• Main Authors: Yuan, Wen-Peng, Liu, Bing, Liu, Chang-Heng, Wang, Xiao-Jun, Zhang, Mian-Song, Meng, Xiu-Mei, Xia, Xue-Kui
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Cell Line; Chitin - therapeutic use; Chitosan - pharmacology; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - pathology; Glutathione Peroxidase - blood; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - pathology; Islets of Langerhans - drug effects; Islets of Langerhans - physiology; Male; Malondialdehyde - blood; Metformin - therapeutic use; Mice; Mice, Inbred NOD; Oligosaccharides - therapeutic use; Original; Rats; Rats, Wistar; Streptozocin - pharmacology; Superoxide Dismutase - blood; 丙二醛含量; 壳寡糖; 总抗氧化能力; 抗氧化活性; 糖尿病大鼠; 胰岛细胞; 谷胱甘肽过氧化物酶; 超氧化物歧化酶活性
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.1339

AIM： To investigate the antioxidant activity of chitooligosaccharides （COSs） on pancreatic islet cells in diabetic rats induced by streptozotocin. METHODS： The antioxidant effect of COSs on pancreatic islet cells was detected under optical microscopy and with colorimetric assay and gel electrophoresis. The activities of glutathione peroxidase and superoxide dismutase, total antioxidant capacity, and content of malondialdehyde in serum and tissue slices of pancreas were examined after 60 d to determine the effect of COSs in streptozotocin-induced diabetes in rats. RESULTS： COSs can prohibit the apoptosis of pancreatic islet cells. All concentrations of COSs can improve the capability of total antioxidant capacity and activity of superoxide dismutase and decrease the content of malondialdehyde drastically. Morphological investigation in the pancreas showed that COSs have resulted in the reduction of islets, loss of pancreatic cells, and nuclear pyknosis of pancreatic cells. CONCLUSION： COSs possess various biological activities and can be used in the treatment of diabetes mellitus.