Structure of Debaryomyces castellii CBS 2923 phytase

Phytate (myo‐inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo‐inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces cas...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2009-04, Vol.65 (4), p.321-326
Main Authors: Ragon, M., Hoh, F., Aumelas, A., Chiche, L., Moulin, G., Boze, H.
Format: Article
Language:English
Subjects:
6-Phytase - chemistry
Acid Phosphatase - chemistry
Amino Acid Sequence
Biochemistry, Molecular Biology
Crystallography, X-Ray
Debaryomyces
Debaryomyces castellii
Hydrolysis
Life Sciences
Models, Molecular
Molecular Sequence Data
phytases
Phytic Acid - chemistry
Protein Structure, Secondary
Saccharomycetales - enzymology
Sequence Alignment
Structural Biology
Structural Communications
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Summary:Phytate (myo‐inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo‐inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 Å resolution. The crystals belonged to space group P6522, with unit‐cell parameters a = 121.65, c = 332.24 Å. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (Rfree = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.
ISSN:1744-3091
1744-3091
DOI:10.1107/S1744309109008653