Influence of Arabidopsis thaliana accessions on rhizobacterial communities and natural variation in root exudates

• Published in: Journal of experimental botany 2009-04, Vol.60 (6), p.1729-1742
• Main Authors: Micallef, Shirley A., Shiaris, Michael P., Colón-Carmona, Adán
• Format: Article
• Language: English
• Subjects: Acid soils; Arabidopsis - metabolism; Arabidopsis - microbiology; Arabidopsis accessions; Arabidopsis thaliana; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Molecular Sequence Data; multi-dimensional scaling; natural variation; Phylogeny; Plant Exudates - metabolism; Plant roots; Plant Roots - metabolism; Plant Roots - microbiology; Plants; Polymerase chain reaction; RESEARCH PAPER; Research Papers; rhizobacterial communities; Rhizosphere; RNA, Ribosomal, 16S - genetics; Root exudates; Soil bacteria; Soil biochemistry; Soil Microbiology; Soil microorganisms
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erp053

Plant species is considered to be one of the most important factors in shaping rhizobacterial communities, but specific plant-microbe interactions in the rhizosphere are still not fully understood. Arabidopsis thaliana, for which a large number of naturally occurring ecotype accessions exist, lacks mycorrhizal associations and is hence an ideal model for rhizobacterial studies. Eight Arabidopsis accessions were found to exert a marked selective influence on bacteria associated with their roots, as determined by terminal-restriction fragment length polymorphism (T-RFLP) and ribosomal intergenic spacer analysis (RISA). Community differences in species composition and relative abundance were both significant (P