Microfluidic Device Architecture for Electrochemical Patterning and Detection of Multiple DNA Sequences

Electrochemical biosensors pose an attractive solution for point-of-care diagnostics because they require minimal instrumentation and they are scalable and readily integrated with microelectronics. The integration of electrochemical biosensors with microscale devices has, however, proven to be chall...

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Bibliographic Details
Published in:Langmuir 2008-02, Vol.24 (3), p.1102-1107
Main Authors: Pavlovic, Elizabeth, Lai, Rebecca Y, Wu, Ting Ting, Ferguson, Brian S, Sun, Ren, Plaxco, Kevin W, Soh, H. T
Format: Article
Language:English
Subjects:
Base Sequence
Biosensing Techniques
Chemistry
Colloidal state and disperse state
DNA - genetics
DNA Probes - genetics
DNA, Viral - genetics
Electrochemistry
Electrochemistry - instrumentation
Electrochemistry - methods
Equipment Design
Exact sciences and technology
General and physical chemistry
Influenza A Virus, H1N1 Subtype - genetics
Influenza A Virus, H5N1 Subtype - genetics
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Polymerase Chain Reaction
Sequence Analysis, DNA - instrumentation
Sequence Analysis, DNA - methods
Surface physical chemistry
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Summary:Electrochemical biosensors pose an attractive solution for point-of-care diagnostics because they require minimal instrumentation and they are scalable and readily integrated with microelectronics. The integration of electrochemical biosensors with microscale devices has, however, proven to be challenging due to significant incompatibilities among biomolecular stability, operation conditions of electrochemical sensors, and microfabrication techniques. Toward a solution to this problem, we have demonstrated here an electrochemical array architecture that supports the following processes in situ, within a self-enclosed microfluidic device:  (a) electrode cleaning and preparation, (b) electrochemical addressing, patterning, and immobilization of sensing biomolecules at selected sensor pixels, (c) sequence-specific electrochemical detection from multiple pixels, and (d) regeneration of the sensing pixels. The architecture we have developed is general, and it should be applicable to a wide range of biosensing schemes that utilize gold−thiol self-assembled monolayer chemistry. As a proof-of-principle, we demonstrate the detection and differentiation of polymerase chain reaction (PCR) amplicons diagnostic of human (H1N1) and avian (H5N1) influenza.
ISSN:0743-7463
1520-5827
DOI:10.1021/la702681c