Isolation and Characterization of a Single-Stranded RNA Virus Infecting the Bloom-Forming Diatom Chaetoceros socialis

Diatoms are very significant primary producers in the world's oceans. Various environmental factors affect the depletion of diatom populations. The importance of viruses as a potential mortality source has recently been recognized. We isolated and characterized a new diatom virus (Chaetoceros s...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2009-04, Vol.75 (8), p.2375-2381
Main Authors: Tomaru, Yuji, Takao, Yoshitake, Suzuki, Hidekazu, Nagumo, Tamotsu, Nagasaki, Keizo
Format: Article
Language:English
Subjects:
Algae
Amino acid sequence
Biological and medical sciences
Cells
Chaetoceros
Cluster Analysis
Diatoms - virology
Fundamental and applied biological sciences. Psychology
Japan
Microbial Ecology
Microbiology
Microscopy, Electron, Transmission
Molecular Sequence Data
Molecular Weight
Open Reading Frames
Phylogeny
RNA polymerase
RNA Viruses - classification
RNA Viruses - genetics
RNA Viruses - isolation & purification
RNA Viruses - ultrastructure
RNA, Viral - genetics
Seawater - virology
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Survival analysis
Viral Proteins - chemistry
Viral Proteins - genetics
Virion - ultrastructure
Viruses
Viruses, Unclassified - classification
Viruses, Unclassified - genetics
Viruses, Unclassified - isolation & purification
Viruses, Unclassified - ultrastructure
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Summary:Diatoms are very significant primary producers in the world's oceans. Various environmental factors affect the depletion of diatom populations. The importance of viruses as a potential mortality source has recently been recognized. We isolated and characterized a new diatom virus (Chaetoceros socialis f. radians RNA virus [CsfrRNAV]) causing the lysis of the bloom-forming species Chaetoceros socialis Lauder f. radians (Schütt) Proschkina-Lavrenko. The virus infectious to C. socialis f. radians was isolated from water samples collected in Hiroshima Bay. Here we show the physiology, morphology, and genome characteristics of the virus clone. Virions were 22 nm in diameter and accumulated in the cytoplasm of the host cells. The latent period and the burst size were estimated to be
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.02580-08