A role for ubiquitin in the clearance of nonfunctional rRNAs

Quality control mechanisms operate in various steps of ribosomal biogenesis to ensure the production of functional ribosome particles. It was reported previously that mature ribosome particles containing nonfunctional mutant rRNAs are also recognized and selectively removed by a cellular quality con...

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Bibliographic Details
Published in:Genes & development 2009-04, Vol.23 (8), p.963-974
Main Authors: Fujii, Kotaro, Kitabatake, Makoto, Sakata, Tomoko, Miyata, Atsumi, Ohno, Mutsuhito
Format: Article
Language:English
Subjects:
Cullin Proteins - metabolism
Cytoplasm - metabolism
Mutation
Research Paper
Ribosome Subunits, Large, Eukaryotic - metabolism
RNA Stability - physiology
RNA, Ribosomal - metabolism
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Ubiquitin - metabolism
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Summary:Quality control mechanisms operate in various steps of ribosomal biogenesis to ensure the production of functional ribosome particles. It was reported previously that mature ribosome particles containing nonfunctional mutant rRNAs are also recognized and selectively removed by a cellular quality control system (nonfunctional rRNA decay [NRD]). Here, we show that the NRD of 25S rRNA requires a ubiquitin E3 ligase component Rtt101p and its associated protein Mms1p, identified previously as factors involved in DNA repair. We revealed that a group of proteins associated with nonfunctional ribosome particles are ubiquitinated in a Rtt101-Mms1-dependent manner. 25S NRD was disrupted when ubiquitination was inhibited by the overexpression of modified ubiquitin molecules, demonstrating a direct role for ubiquitin in this pathway. These results uncovered an unexpected connection between DNA repair and the quality control of rRNAs. Our findings support a model in which responses to DNA and rRNA damages are triggered by a common ubiquitin ligase complex during genotoxic stress harmful to both molecules.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1775609