Messenger RNA for neuropeptide Y in the arcuate nucleus increases in parallel with plasma adrenocorticotropin during sepsis in the rat

Loss of appetite occurs in the cecal ligation and puncture (CLP) model of sepsis in conjunction with the activation of central neural stress pathways. Neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus is upregulated by several stressors and is stimulatory to feeding. To examine the res...

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Bibliographic Details
Published in:Neuroscience letters 2009-03, Vol.452 (2), p.146-150
Main Authors: Carlson, Drew E., Le, WeiWei, Chiu, William C., Hoffman, Gloria E.
Format: Article
Language:English
Subjects:
Adrenal Cortex - metabolism
Adrenal Cortex - secretion
Adrenal Cortex Hormones - metabolism
Adrenal Cortex Hormones - secretion
Adrenocorticotropic hormone
Adrenocorticotropic Hormone - blood
Adrenocorticotropic Hormone - secretion
Adrenocorticotropin
Animals
Appetite - physiology
Arcuate nucleus
Arcuate Nucleus of Hypothalamus - cytology
Arcuate Nucleus of Hypothalamus - metabolism
Arcuate Nucleus of Hypothalamus - secretion
Biological and medical sciences
Cecal ligation and puncture
Disease Models, Animal
Feeding Behavior - physiology
Fundamental and applied biological sciences. Psychology
In situ hybridization
Male
Neurons - metabolism
Neurons - secretion
Neuropeptide Y
Neuropeptide Y - genetics
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Sepsis
Sepsis - blood
Sepsis - genetics
Sepsis - physiopathology
Stress, Physiological - physiology
Up-Regulation - physiology
Vertebrates: nervous system and sense organs
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Summary:Loss of appetite occurs in the cecal ligation and puncture (CLP) model of sepsis in conjunction with the activation of central neural stress pathways. Neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus is upregulated by several stressors and is stimulatory to feeding. To examine the response of NPY messenger RNA in the arcuate nucleus to sepsis, we used biotinylated RNA probes and a quantitative non-isotopic in situ hybridization approach in cryo-preserved sections from rats made septic by CLP. The mRNA in arcuate neurons was upregulated from the first day after CLP. By the afternoon of the third day through the morning of the fourth day, the average grey level of NPY mRNA clusters was 30% greater after CLP than after sham surgery (P
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2009.01.046