Cytology and receptor architecture of human anterior cingulate cortex

• Published in: Journal of comparative neurology (1911) 2008-06, Vol.508 (6), p.906-926
• Main Authors: Palomero-Gallagher, Nicola, Mohlberg, Hartmut, Zilles, Karl, Vogt, Brent
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; autoradiography; Autoradiography - methods; Brain Mapping; Cell Biology; cingulate motor area; cortical layers; cytoarchitecture; Female; Gyrus Cinguli - anatomy & histology; Gyrus Cinguli - metabolism; Gyrus Cinguli - ultrastructure; Humans; Male; Middle Aged; Models, Neurological; neurofilament proteins; neurotransmitter; Postmortem Changes; Receptors, Neurotransmitter - classification; Receptors, Neurotransmitter - metabolism; Receptors, Neurotransmitter - ultrastructure; Silver Staining - methods
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.21684

Anterior cingulate cortex (ACC) is involved in emotion, mood, and autonomic regulation. Although a subgenual part of ACC (sACC) may be vulnerable in depression and area 25 is cytologically unique, there are no assessments that contrast this region with pregenual ACC (pACC). Thus, we undertook independent multimodal verifications of architectural differences among subregions and areas. Areas 24a and 24b have pregenual and subgenual components. The latter have a thin layer III. Area 24c has dorsal (pd24c) and ventral (pv24c) parts. Area pd24c has larger neurofilament‐expressing neurons in layer Va, and neurons in Vb form aggregates in area pv24c. Area pd24c occupies both banks of the cingulate sulcus, with pv24c on the ventral bank. Layer III of pd24cd has many larger neurofilament‐expressing neurons and a richer dendritic plexus. Area 32 has pregenual (p32) and subgenual (s32) components. Layer II in s32 is of particular note because it has a neuron‐dense IIa and sparse IIb. Area 25 has anterior (25a) and posterior (25p) parts; 25p has the thinnest layer III in the cingulate gyrus. Area 25a contains significantly higher AMPA, kainate, NMDA, GABAA, GABAB, and α1 densities than 25p. Area 33 continues around the genu and ventrally to encompass the full caudal extent of area 25. Subgenual ACC has significantly higher GABAA, GABAB, benzodiazepine (BZ), α1, and 5‐HT1A densities than pACC. GABAB, BZ, and α1 binding confirms the subdivision of area pd24c. In conclusion, ACC comprises two parts that are unique in terms of their cytoarchitecture and neurotransmitter receptor organization. J. Comp. Neurol. 508:906–926, 2008. © 2008 Wiley‐Liss, Inc.