A single C. elegans PUF protein binds RNA in multiple modes

PUF proteins specifically bind mRNAs to regulate their stability and translation. Here we focus on the RNA-binding specificity of a C. elegans PUF protein, PUF-11. Our findings reveal that PUF-11 binds RNA in multiple modes, in which the protein can accommodate variable spacings between two distinct...

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Bibliographic Details
Published in:RNA (Cambridge) 2009-06, Vol.15 (6), p.1090-1099
Main Authors: Koh, Yvonne Yiling, Opperman, Laura, Stumpf, Craig, Mandan, Arpita, Keles, Sunduz, Wickens, Marvin
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - metabolism
Models, Biological
Models, Molecular
Protein Conformation
RNA - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
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Summary:PUF proteins specifically bind mRNAs to regulate their stability and translation. Here we focus on the RNA-binding specificity of a C. elegans PUF protein, PUF-11. Our findings reveal that PUF-11 binds RNA in multiple modes, in which the protein can accommodate variable spacings between two distinct recognition elements. We propose a structural model in which flexibility in the central region of the protein enables the protein to adopt at least two distinct structures, one of which results in base flipping.
ISSN:1355-8382
1469-9001
DOI:10.1261/rna.1545309