Human Gene and Protein Database (HGPD): a novel database presenting a large quantity of experiment-based results in human proteomics

Completion of human genome sequencing has greatly accelerated functional genomic research. Full-length cDNA clones are essential experimental tools for functional analysis of human genes. In one of the projects of the New Energy and Industrial Technology Development Organization (NEDO) in Japan, the...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 2009-01, Vol.37 (suppl-1), p.D762-D766
Main Authors: Maruyama, Yukio, Wakamatsu, Ai, Kawamura, Yoshifumi, Kimura, Kouichi, Yamamoto, Jun-ichi, Nishikawa, Tetsuo, Kisu, Yasutomo, Sugano, Sumio, Goshima, Naoki, Isogai, Takao, Nomura, Nobuo
Format: Article
Language:English
Subjects:
Cloning, Molecular
Databases, Protein
DNA, Complementary - chemistry
Electrophoresis, Polyacrylamide Gel
Genes
Humans
Internet
Protein Biosynthesis
Proteins - chemistry
Proteins - genetics
Proteins - metabolism
Proteomics
Triticum aestivum
User-Computer Interface
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Completion of human genome sequencing has greatly accelerated functional genomic research. Full-length cDNA clones are essential experimental tools for functional analysis of human genes. In one of the projects of the New Energy and Industrial Technology Development Organization (NEDO) in Japan, the full-length human cDNA sequencing project (FLJ project), nucleotide sequences of approximately 30 000 human cDNA clones have been analyzed. The Gateway system is a versatile framework to construct a variety of expression clones for various experiments. We have constructed 33 275 human Gateway entry clones from full-length cDNAs, representing to our knowledge the largest collection in the world. Utilizing these clones with a highly efficient cell-free protein synthesis system based on wheat germ extract, we have systematically and comprehensively produced and analyzed human proteins in vitro. Sequence information for both amino acids and nucleotides of open reading frames of cDNAs cloned into Gateway entry clones and in vitro expression data using those clones can be retrieved from the Human Gene and Protein Database (HGPD, http://www.HGPD.jp). HGPD is a unique database that stores the information of a set of human Gateway entry clones and protein expression data and helps the user to search the Gateway entry clones.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkn872