Molecular structure of the largemouth bass (Micropterus salmoides) Myf5 gene and its effect on skeletal muscle growth

Myogenic Regulatory Factors (MRFs), a family of basic helix-loop-helix (bHLH) transcription factors, play important roles in regulating skeletal muscle development and growth. Myf5, the primary factor of MRFs, initiates myogenesis. Its expression pattern during somitomyogenesis in some fish has been...

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Bibliographic Details
Published in:Molecular biology reports 2009-07, Vol.36 (6), p.1497-1504
Main Authors: Guo, Yuhan, Bai, Junjie, Chang, Ouqin, Lao, Haihua, Ye, Xing, Luo, Jianren
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Base Sequence
Bass - genetics
Biomedical and Life Sciences
Cloning
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA, Complementary - administration & dosage
DNA, Complementary - genetics
DNA, Complementary - pharmacology
Fish
Freshwater
Genes
Histology
Hypertrophy - chemically induced
Life Sciences
Micropterus salmoides
Molecular biology
Morphology
Muscle Development
Muscle, Skeletal - drug effects
Muscle, Skeletal - growth & development
Myofibrils
Myogenic Regulatory Factor 5 - administration & dosage
Myogenic Regulatory Factor 5 - genetics
Myogenic Regulatory Factor 5 - physiology
Tilapia
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Summary:Myogenic Regulatory Factors (MRFs), a family of basic helix-loop-helix (bHLH) transcription factors, play important roles in regulating skeletal muscle development and growth. Myf5, the primary factor of MRFs, initiates myogenesis. Its expression pattern during somitomyogenesis in some fish has been revealed. To further study its effect on fish muscle during postembryonic growth, characterization and function analysis of myf5 cDNA were carried out in largemouth bass. The 1,093 bp cDNA sequence was identified by RT-PCR and 3'RACE, then the ORF of Myf5 cDNA was cloned into the expression vector pcDNA3.1(-)/mycHisB. The recombinant plasmid pcDNA3.1(-)/mycHisB-Myf5 was injected into the dorsal muscle of tilapias. RT-PCR and histochemical results showed that the exogenous gene was transcribed and translated in vivo. Its effect on muscle growth focused on myofiber hypertrophy in white muscle 60 days post injection. This indicated that overexpression of Myf5 can promote myogenesis during the fish muscle postembryonic growth period.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-008-9341-1