Localization of glutamate receptors to distal dendrites depends on subunit composition and the kinesin motor protein KIF17

Correct glutamate receptor localization in neurons is crucial for neurotransmission in the brain. Here we investigated the mechanisms underlying localization of kainate GluR5 receptors to dendrites in cultured hippocampal neurons. We find that the GluR5 distribution depends on association with GluR6...

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Bibliographic Details
Published in:Molecular and cellular neuroscience 2007-02, Vol.34 (2), p.219-230
Main Authors: Kayadjanian, N., Lee, H.S., Piña-Crespo, J., Heinemann, S.F.
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Cultured hippocampal neurons
Dendrites - metabolism
Embryo, Mammalian
GluR5
GluR6 and KA2
Green Fluorescent Proteins - metabolism
Hippocampus - cytology
Immunohistochemistry - methods
Immunoprecipitation - methods
Kainate receptor knock-out mice
Kainate receptors
KIF17 motor proteins
Kinesin - metabolism
Mice
Mice, Knockout
Microtubule motor protein
Mutagenesis - physiology
Neurons - cytology
Protein Subunits - metabolism
Proximal and distal dendrites
Rats
Rats, Long-Evans
Receptor trafficking
Receptors, Kainic Acid - deficiency
Receptors, Kainic Acid - metabolism
Subunit composition
Transfection - methods
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Summary:Correct glutamate receptor localization in neurons is crucial for neurotransmission in the brain. Here we investigated the mechanisms underlying localization of kainate GluR5 receptors to dendrites in cultured hippocampal neurons. We find that the GluR5 distribution depends on association with GluR6 and KA2 subunits. The GluR5 subunit was expressed in distal dendrites only when GluR6 and KA2 subunits were present, whereas it was restricted to proximal dendrites in the absence of these subunits. The overlap between GluR5 distribution and the organization of microtubules in dendrites led us to examine whether KIF17, a microtubule motor protein expressed in distal dendrites, is involved in GluR5 localization to distal dendrites. We show here, for the first time that the microtubule motor protein KIF17 interacts with GluR6 and KA2 subunits and is required for GluR5 localization to distal dendrites, defining a novel mechanism that controls receptor localization in neurons.
ISSN:1044-7431
1095-9327
DOI:10.1016/j.mcn.2006.11.001