Cre-mediated recombination in pituitary somatotropes

We report a transgenic line with highly penetrant cre recombinase activity in the somatotrope cells of the anterior pituitary gland. Expression of the cre transgene is under the control of the locus control region of the human growth hormone gene cluster and the rat growth hormone promoter. Cre reco...

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2009-01, Vol.47 (1), p.55-60
Main Authors: Nasonkin, Igor O., Potok, Mary Anne, Camper, Sally A.
Format: Article
Language:English
Subjects:
Animals
anterior pituitary gland
Cell Line
cell specific expression
Cytomegalovirus
Embryo, Mammalian - embryology
Embryo, Mammalian - metabolism
Genetic Engineering - methods
growth hormone
Growth Hormone - genetics
Growth Hormone - metabolism
Humans
Integrases - genetics
Integrases - metabolism
Mice
prolactin
Rats
Recombination, Genetic - genetics
Somatotrophs - cytology
Somatotrophs - metabolism
transgene
Transgenes - genetics
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Summary:We report a transgenic line with highly penetrant cre recombinase activity in the somatotrope cells of the anterior pituitary gland. Expression of the cre transgene is under the control of the locus control region of the human growth hormone gene cluster and the rat growth hormone promoter. Cre recombinase activity was assessed with two different lacZ reporter genes that require excision of a floxed stop sequence for expression: a chick β‐actin promoter with the CMV enhancer transgene and a ROSA26 knock‐in. Cre activity is detectable in the developing pituitary after initiation of Gh transcription and persists through adulthood with high penetrance in Gh expressing cells and lower penetrance in lactotropes, a cell type that shares a common origin with somatotropes. This Gh‐cre transgenic line is suitable for efficient, cell‐specific deletion of floxed regions of genomic DNA in differentiated somatotropes and a subset of lactotrope cells of the anterior pituitary gland. genesis 47:55–60, 2009. © 2008 Wiley‐Liss, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/dvg.20462