ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting

Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investi...

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Bibliographic Details
Published in:The Journal of cell biology 2009-04, Vol.185 (2), p.213-224
Main Authors: Shields, S. Brookhart, Oestreich, Andrea J, Winistorfer, Stanley, Nguyen, Doris, Payne, Johanna A, Katzmann, David J, Piper, Robert
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Binding sites
Biochemistry
Carboxypeptidases - genetics
Carboxypeptidases - metabolism
Endosomal Sorting Complexes Required for Transport
Endosomes - metabolism
Experiments
Freight
Genetic mutation
Glues
Membrane Proteins - metabolism
Membranes
Models, Molecular
Molecular Sequence Data
Multiprotein Complexes - metabolism
Multivesicular bodies
Plasmids
Protein Binding
Protein Structure, Tertiary
Protein Transport - physiology
Proteins
Receptors
Receptors, Mating Factor - genetics
Receptors, Mating Factor - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Ubiquitin - metabolism
Ubiquitins
Vesicular Transport Proteins - genetics
Vesicular Transport Proteins - metabolism
Yeasts
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Summary:Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200811130