Changes in the subcellular distribution of NADPH oxidase subunit p47phox in dendrites of rat dorsomedial nucleus tractus solitarius neurons in response to chronic administration of hypertensive agents

NADPH oxidase-generated superoxide can modulate crucial intracellular signaling cascades in neurons of the nucleus tractus solitarius (NTS), a brain region that plays an important role in cardiovascular processes. Modulation of NTS signaling by superoxide may be linked to the subcellular location of...

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Bibliographic Details
Published in:Experimental neurology 2007-06, Vol.205 (2), p.383-395
Main Authors: GLASS, Michael J, CHAN, June, FRYS, Kelly A, OSELKIN, Martin, TARSITANO, M. Jacqueline, IADECOLA, Costantino, PICKEL, Virginia M
Format: Article
Language:English
Subjects:
Angiotensin II - pharmacology
Animals
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Blood and lymphatic vessels
Blood Pressure - drug effects
Cardiology. Vascular system
Dendrites - enzymology
Experimental diseases
Hypertension - chemically induced
Immunoenzyme Techniques
Immunohistochemistry
Male
Medical sciences
Microscopy, Electron
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
NADPH Oxidases - metabolism
Neurology
Neurons - enzymology
Phenylephrine - pharmacology
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1 - metabolism
Solitary Nucleus - cytology
Solitary Nucleus - enzymology
Subcellular Fractions - enzymology
Subcellular Fractions - ultrastructure
Sympathomimetics - pharmacology
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Summary:NADPH oxidase-generated superoxide can modulate crucial intracellular signaling cascades in neurons of the nucleus tractus solitarius (NTS), a brain region that plays an important role in cardiovascular processes. Modulation of NTS signaling by superoxide may be linked to the subcellular location of the mobile NADPH oxidase p47(phox) subunit, which is known to be present in dendrites of NTS neurons. It is not known, however, if hypertension can produce changes in the trafficking of p47(phox) in defined NTS subregions, particularly the preferentially barosensitive dorsomedial NTS (dmNTS), or preferentially gastrointestinal medial NTS (mNTS). We used immunogold electron microscopy to determine if p47(phox) localization was differentially affected in dendritic profiles of neurons from these NTS subregions of the rat in response to distinct models of hypertension, namely chronic 7-day subcutaneous administration of angiotensin II (AngII), or phenylephrine. In small (
ISSN:0014-4886
1090-2430
DOI:10.1016/j.expneurol.2007.02.016