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Adhesions in a murine flexor tendon graft model: Autograft versus allograft reconstruction
Reconstruction of flexor tendons often results in adhesions that compromise joint flexion. Little is known about the factors involved in the formation of flexor tendon graft adhesions. In this study, we developed and characterized a novel mouse model of flexor digitorum longus (FDL) tendon reconstru...
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| Published in: | Journal of orthopaedic research 2008-06, Vol.26 (6), p.824-833 |
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| creator | Hasslund, Sys Jacobson, Justin A. Dadali, Tulin Basile, Patrick Ulrich-Vinther, Michael Søballe, Kjeld Schwarz, Edward M. O'Keefe, Regis J. Mitten, David J. Awad, Hani A. |
| description | Reconstruction of flexor tendons often results in adhesions that compromise joint flexion. Little is known about the factors involved in the formation of flexor tendon graft adhesions. In this study, we developed and characterized a novel mouse model of flexor digitorum longus (FDL) tendon reconstruction with live autografts or reconstituted freeze‐dried allografts. Grafted tendons were evaluated at multiple time points up to 84 days post‐reconstruction. To assess the flexion range of the metatarsophalangeal joint, we developed a quantitative outcome measure proportional to the resistance to tendon gliding due to adhesions, which we termed the Gliding Coefficient. At 14 days post‐grafting, the Gliding Coefficient was 29‐ and 26‐fold greater than normal FDL tendon for both autografts and allografts, respectively (p |
| doi_str_mv | 10.1002/jor.20531 |
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Little is known about the factors involved in the formation of flexor tendon graft adhesions. In this study, we developed and characterized a novel mouse model of flexor digitorum longus (FDL) tendon reconstruction with live autografts or reconstituted freeze‐dried allografts. Grafted tendons were evaluated at multiple time points up to 84 days post‐reconstruction. To assess the flexion range of the metatarsophalangeal joint, we developed a quantitative outcome measure proportional to the resistance to tendon gliding due to adhesions, which we termed the Gliding Coefficient. At 14 days post‐grafting, the Gliding Coefficient was 29‐ and 26‐fold greater than normal FDL tendon for both autografts and allografts, respectively (p < 0.001), and subsequently doubled for 28‐day autografts. Interestingly, there were no significant differences in maximum tensile force or stiffness between live autograft and freeze‐dried allograft repairs over time. Histologically, autograft healing was characterized by extensive remodeling and exuberant scarring around both the ends and the body of the graft, whereas allograft scarring was abundant only near the graft–host junctions. Gene expression of GDF‐5 and VEGF were significantly increased in 28‐day autografts compared to allografts and to normal tendons. These results suggest that the biomechanical advantages for tendon reconstruction using live autografts over devitalized allografts are minimal. This mouse model can be useful in elucidating the molecular mechanisms in tendon repair and can aid in preliminary screening of molecular treatments of flexor tendon adhesions. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:824–833, 2008</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.20531</identifier><identifier>PMID: 18186128</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Achilles Tendon - pathology ; Achilles Tendon - physiology ; Achilles Tendon - transplantation ; Actins - genetics ; adhesion ; allograft ; Animals ; autograft ; Biomechanical Phenomena ; biomechanics ; Bone Morphogenetic Proteins - genetics ; flexor tendon ; Freeze Drying ; Gene Expression ; Growth Differentiation Factor 5 ; Metatarsophalangeal Joint - physiology ; Mice ; Mice, Inbred C57BL ; Models, Animal ; Orthopedic Procedures - methods ; Range of Motion, Articular ; Reconstructive Surgical Procedures - methods ; Tissue Adhesions - pathology ; Tissue Adhesions - physiopathology ; Tissue Adhesions - prevention & control ; Transforming Growth Factor beta1 - genetics ; Transplantation, Autologous ; Transplantation, Homologous ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Journal of orthopaedic research, 2008-06, Vol.26 (6), p.824-833</ispartof><rights>Copyright © 2008 Orthopaedic Research Society</rights><rights>(c) 2008 Orthopaedic Research Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4511-2a5b51f8adb1387eb1d58e7a43e2e07a4ba13fe5b65a856c90075847048171433</citedby><cites>FETCH-LOGICAL-c4511-2a5b51f8adb1387eb1d58e7a43e2e07a4ba13fe5b65a856c90075847048171433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18186128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasslund, Sys</creatorcontrib><creatorcontrib>Jacobson, Justin A.</creatorcontrib><creatorcontrib>Dadali, Tulin</creatorcontrib><creatorcontrib>Basile, Patrick</creatorcontrib><creatorcontrib>Ulrich-Vinther, Michael</creatorcontrib><creatorcontrib>Søballe, Kjeld</creatorcontrib><creatorcontrib>Schwarz, Edward M.</creatorcontrib><creatorcontrib>O'Keefe, Regis J.</creatorcontrib><creatorcontrib>Mitten, David J.</creatorcontrib><creatorcontrib>Awad, Hani A.</creatorcontrib><title>Adhesions in a murine flexor tendon graft model: Autograft versus allograft reconstruction</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>Reconstruction of flexor tendons often results in adhesions that compromise joint flexion. Little is known about the factors involved in the formation of flexor tendon graft adhesions. In this study, we developed and characterized a novel mouse model of flexor digitorum longus (FDL) tendon reconstruction with live autografts or reconstituted freeze‐dried allografts. Grafted tendons were evaluated at multiple time points up to 84 days post‐reconstruction. To assess the flexion range of the metatarsophalangeal joint, we developed a quantitative outcome measure proportional to the resistance to tendon gliding due to adhesions, which we termed the Gliding Coefficient. At 14 days post‐grafting, the Gliding Coefficient was 29‐ and 26‐fold greater than normal FDL tendon for both autografts and allografts, respectively (p < 0.001), and subsequently doubled for 28‐day autografts. Interestingly, there were no significant differences in maximum tensile force or stiffness between live autograft and freeze‐dried allograft repairs over time. Histologically, autograft healing was characterized by extensive remodeling and exuberant scarring around both the ends and the body of the graft, whereas allograft scarring was abundant only near the graft–host junctions. Gene expression of GDF‐5 and VEGF were significantly increased in 28‐day autografts compared to allografts and to normal tendons. These results suggest that the biomechanical advantages for tendon reconstruction using live autografts over devitalized allografts are minimal. This mouse model can be useful in elucidating the molecular mechanisms in tendon repair and can aid in preliminary screening of molecular treatments of flexor tendon adhesions. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:824–833, 2008</description><subject>Achilles Tendon - pathology</subject><subject>Achilles Tendon - physiology</subject><subject>Achilles Tendon - transplantation</subject><subject>Actins - genetics</subject><subject>adhesion</subject><subject>allograft</subject><subject>Animals</subject><subject>autograft</subject><subject>Biomechanical Phenomena</subject><subject>biomechanics</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>flexor tendon</subject><subject>Freeze Drying</subject><subject>Gene Expression</subject><subject>Growth Differentiation Factor 5</subject><subject>Metatarsophalangeal Joint - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Models, Animal</subject><subject>Orthopedic Procedures - methods</subject><subject>Range of Motion, Articular</subject><subject>Reconstructive Surgical Procedures - methods</subject><subject>Tissue Adhesions - pathology</subject><subject>Tissue Adhesions - physiopathology</subject><subject>Tissue Adhesions - prevention & control</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Transplantation, Autologous</subject><subject>Transplantation, Homologous</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp1kUFPFTEQgBujkSd48A-Ynkw8LHTa7bZ4MHkhghACCYFovDTd3VkodrfQ7iL8ewv7BDlwmmb6zTfTDiEfgG0CY3zrMsRNzqSAV2QBUpaF5Orna7JgSlQF41W1Rt6ldMkYU8D1W7IGGnSVjwvya9leYHJhSNQN1NJ-im5A2nm8DZGOOLRhoOfRdiPtQ4v-C11OY5gTNxjTlKj1fpWI2GTRGKdmzMYN8qazPuH7VVwnZ7vfTne-F4fHe_s7y8OiKSVAwa2sJXTatjUIrbCGVmpUthTIkeVYWxAdyrqSVsuq2c6vkLpUrNSgoBRinXydvVdT3WPb4DBG681VdL2NdyZYZ57fDO7CnIcbwxXb5rrKgk8rQQzXE6bR9C416L0dMEzJqNwQJNyDn2ewiSGliN1jE2DmfhMmb8I8bCKzH_-f6olcfX0Gtmbgj_N497LJHByf_FMWc4VLI94-Vtj421RKKGl-HO2Z8khIONW75kD8BSNNo5M</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Hasslund, Sys</creator><creator>Jacobson, Justin A.</creator><creator>Dadali, Tulin</creator><creator>Basile, Patrick</creator><creator>Ulrich-Vinther, Michael</creator><creator>Søballe, Kjeld</creator><creator>Schwarz, Edward M.</creator><creator>O'Keefe, Regis J.</creator><creator>Mitten, David J.</creator><creator>Awad, Hani A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200806</creationdate><title>Adhesions in a murine flexor tendon graft model: Autograft versus allograft reconstruction</title><author>Hasslund, Sys ; Jacobson, Justin A. ; Dadali, Tulin ; Basile, Patrick ; Ulrich-Vinther, Michael ; Søballe, Kjeld ; Schwarz, Edward M. ; O'Keefe, Regis J. ; Mitten, David J. ; Awad, Hani A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4511-2a5b51f8adb1387eb1d58e7a43e2e07a4ba13fe5b65a856c90075847048171433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Achilles Tendon - pathology</topic><topic>Achilles Tendon - physiology</topic><topic>Achilles Tendon - transplantation</topic><topic>Actins - genetics</topic><topic>adhesion</topic><topic>allograft</topic><topic>Animals</topic><topic>autograft</topic><topic>Biomechanical Phenomena</topic><topic>biomechanics</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>flexor tendon</topic><topic>Freeze Drying</topic><topic>Gene Expression</topic><topic>Growth Differentiation Factor 5</topic><topic>Metatarsophalangeal Joint - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Models, Animal</topic><topic>Orthopedic Procedures - methods</topic><topic>Range of Motion, Articular</topic><topic>Reconstructive Surgical Procedures - methods</topic><topic>Tissue Adhesions - pathology</topic><topic>Tissue Adhesions - physiopathology</topic><topic>Tissue Adhesions - prevention & control</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Transplantation, Autologous</topic><topic>Transplantation, Homologous</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasslund, Sys</creatorcontrib><creatorcontrib>Jacobson, Justin A.</creatorcontrib><creatorcontrib>Dadali, Tulin</creatorcontrib><creatorcontrib>Basile, Patrick</creatorcontrib><creatorcontrib>Ulrich-Vinther, Michael</creatorcontrib><creatorcontrib>Søballe, Kjeld</creatorcontrib><creatorcontrib>Schwarz, Edward M.</creatorcontrib><creatorcontrib>O'Keefe, Regis J.</creatorcontrib><creatorcontrib>Mitten, David J.</creatorcontrib><creatorcontrib>Awad, Hani A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasslund, Sys</au><au>Jacobson, Justin A.</au><au>Dadali, Tulin</au><au>Basile, Patrick</au><au>Ulrich-Vinther, Michael</au><au>Søballe, Kjeld</au><au>Schwarz, Edward M.</au><au>O'Keefe, Regis J.</au><au>Mitten, David J.</au><au>Awad, Hani A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adhesions in a murine flexor tendon graft model: Autograft versus allograft reconstruction</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2008-06</date><risdate>2008</risdate><volume>26</volume><issue>6</issue><spage>824</spage><epage>833</epage><pages>824-833</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>Reconstruction of flexor tendons often results in adhesions that compromise joint flexion. Little is known about the factors involved in the formation of flexor tendon graft adhesions. In this study, we developed and characterized a novel mouse model of flexor digitorum longus (FDL) tendon reconstruction with live autografts or reconstituted freeze‐dried allografts. Grafted tendons were evaluated at multiple time points up to 84 days post‐reconstruction. To assess the flexion range of the metatarsophalangeal joint, we developed a quantitative outcome measure proportional to the resistance to tendon gliding due to adhesions, which we termed the Gliding Coefficient. At 14 days post‐grafting, the Gliding Coefficient was 29‐ and 26‐fold greater than normal FDL tendon for both autografts and allografts, respectively (p < 0.001), and subsequently doubled for 28‐day autografts. Interestingly, there were no significant differences in maximum tensile force or stiffness between live autograft and freeze‐dried allograft repairs over time. Histologically, autograft healing was characterized by extensive remodeling and exuberant scarring around both the ends and the body of the graft, whereas allograft scarring was abundant only near the graft–host junctions. Gene expression of GDF‐5 and VEGF were significantly increased in 28‐day autografts compared to allografts and to normal tendons. These results suggest that the biomechanical advantages for tendon reconstruction using live autografts over devitalized allografts are minimal. This mouse model can be useful in elucidating the molecular mechanisms in tendon repair and can aid in preliminary screening of molecular treatments of flexor tendon adhesions. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:824–833, 2008</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18186128</pmid><doi>10.1002/jor.20531</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley |
| subjects | Achilles Tendon - pathology Achilles Tendon - physiology Achilles Tendon - transplantation Actins - genetics adhesion allograft Animals autograft Biomechanical Phenomena biomechanics Bone Morphogenetic Proteins - genetics flexor tendon Freeze Drying Gene Expression Growth Differentiation Factor 5 Metatarsophalangeal Joint - physiology Mice Mice, Inbred C57BL Models, Animal Orthopedic Procedures - methods Range of Motion, Articular Reconstructive Surgical Procedures - methods Tissue Adhesions - pathology Tissue Adhesions - physiopathology Tissue Adhesions - prevention & control Transforming Growth Factor beta1 - genetics Transplantation, Autologous Transplantation, Homologous Vascular Endothelial Growth Factor A - genetics |
| title | Adhesions in a murine flexor tendon graft model: Autograft versus allograft reconstruction |
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