In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy

We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimula...

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Bibliographic Details
Published in:Biophysical journal 2009-01, Vol.96 (1), p.L1-L3
Main Authors: Schröder, Jan, Benink, Hélène, Dyba, Marcus, Los, Georgyi V.
Format: Article
Language:English
Subjects:
Biophysical Letter
Cell Line, Tumor
Cells
Fluorescence
Fluorescent Dyes
Gene Transfer Techniques
Genes, Reporter - genetics
HeLa Cells
Humans
Integrin beta1 - genetics
Integrin beta1 - metabolism
Medical imaging
Membranes
Microscopy
Microscopy - methods
Proteins
Pseudopodia - metabolism
Pseudopodia - ultrastructure
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Summary:We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2008.09.032