Attomole Microarray Detection of MicroRNAs by Nanoparticle-Amplified SPR Imaging Measurements of Surface Polyadenylation Reactions

Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarr...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-11, Vol.128 (43), p.14044-14046
Main Authors: Fang, Shiping, Lee, Hye Jin, Wark, Alastair W, Corn, Robert M
Format: Article
Language:English
Subjects:
Adenosine Monophosphate - chemistry
Chemistry
Exact sciences and technology
General and physical chemistry
MicroRNAs - analysis
Nanoparticles
Oligonucleotide Array Sequence Analysis
Sensitivity and Specificity
Surface Plasmon Resonance - methods
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Summary:Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja065223p