Expressed sequence tags analysis of Blattella germanica

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editin...

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Bibliographic Details
Published in:Korean journal of parasitology 2005-12, Vol.43 (4), p.149-156
Main Authors: Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea), Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea), Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea), Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea), Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea), Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea), Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr, Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)
Format: Article
Language:English
Subjects:
Animals
Base Sequence
BLAST search
BLATTELLA GERMANICA
Blattellidae - genetics
cDNA library
ESTs
Expressed Sequence Tags
Female
Male
Molecular Sequence Data
novel genes
Original
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
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Summary:Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function.
ISSN:0023-4001
1738-0006
DOI:10.3347/kjp.2005.43.4.149