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Expressed sequence tags analysis of Blattella germanica
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editin...
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| Published in: | Korean journal of parasitology 2005-12, Vol.43 (4), p.149-156 |
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| container_end_page | 156 |
| container_issue | 4 |
| container_start_page | 149 |
| container_title | Korean journal of parasitology |
| container_volume | 43 |
| creator | Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea) Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea) Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea) Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea) Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea) Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea) Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea) |
| description | Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. |
| doi_str_mv | 10.3347/kjp.2005.43.4.149 |
| format | article |
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(Kosin University College of Medicine, Busan, Republic of Korea) ; Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea) ; Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea) ; Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr ; Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)</creator><creatorcontrib>Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea) ; Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea) ; Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea) ; Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr ; Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)</creatorcontrib><description>Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function.</description><identifier>ISSN: 0023-4001</identifier><identifier>EISSN: 1738-0006</identifier><identifier>DOI: 10.3347/kjp.2005.43.4.149</identifier><identifier>PMID: 16340304</identifier><language>eng</language><publisher>Korea (South): 대한기생충학열대의학회</publisher><subject>Animals ; Base Sequence ; BLAST search ; BLATTELLA GERMANICA ; Blattellidae - genetics ; cDNA library ; ESTs ; Expressed Sequence Tags ; Female ; Male ; Molecular Sequence Data ; novel genes ; Original ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment</subject><ispartof>Korean journal of parasitology, 2005-12, Vol.43 (4), p.149-156</ispartof><rights>Copyright © 2005 by The Korean Society for Parasitology 2005</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3649-5016bb71bffaa2dc4534d618555f3d26e88dabe95f364f6287f83c4d0f71d29b3</citedby><cites>FETCH-LOGICAL-c3649-5016bb71bffaa2dc4534d618555f3d26e88dabe95f364f6287f83c4d0f71d29b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712019/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2712019/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16340304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr</creatorcontrib><creatorcontrib>Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)</creatorcontrib><title>Expressed sequence tags analysis of Blattella germanica</title><title>Korean journal of parasitology</title><addtitle>Korean J Parasitol</addtitle><description>Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>BLAST search</subject><subject>BLATTELLA GERMANICA</subject><subject>Blattellidae - genetics</subject><subject>cDNA library</subject><subject>ESTs</subject><subject>Expressed Sequence Tags</subject><subject>Female</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>novel genes</subject><subject>Original</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Alignment</subject><issn>0023-4001</issn><issn>1738-0006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpVkE1v1DAQhi0EokvhB3AA5QK3hBmP4zgXJCjLh6iohOBsTWJ7SckmWzuL6L_Hq10VOFnWPPPOzCPEU4SKSDWvfl7vKglQV4oqVaFq74kVNmRKAND3xQpAUqkA8Ew8SukagGTd4ENxhpoUEKiVaNa_d9Gn5F2R_M3eT70vFt6kgiceb9OQijkUb0deFj-OXGx83PI09PxYPAg8Jv_k9J6L7-_X3y4-lpdXHz5dvLkse9KqLWtA3XUNdiEwS9ermpTTaOq6DuSk9sY47nybf1oFLU0TDPXKQWjQybajc_H6mLvbd1vvej8tkUe7i8OW462debD_V6bhh93Mv6xsUAK2OeDlKSDO-b602O2Q-sMxk5_3yWpjTE2aMohHsI9zStGHuyEI9qDbZt32oNsqsspm3bnn-b_b_e04-c3AiyMw7XPJu4HvmC9X79Yg0ai2xcw9O3KBZ8ubOCT7-ethFqDMuugPbsKRpw</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea)</creator><creator>Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea)</creator><creator>Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea)</creator><creator>Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea)</creator><creator>Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea)</creator><creator>Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea)</creator><creator>Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr</creator><creator>Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)</creator><general>대한기생충학열대의학회</general><general>The Korean Society for Parasitology</general><scope>FBQ</scope><scope>DBRKI</scope><scope>TDB</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20051201</creationdate><title>Expressed sequence tags analysis of Blattella germanica</title><author>Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea) ; Yu, H.S. (Pusan National University College of Medicine, Busan, Republic of Korea) ; Yu, T.H. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, B.J. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea) ; Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea) ; Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr ; Jeong, H.J. (Pusan National University College of Medicine, Busan, Republic of Korea)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3649-5016bb71bffaa2dc4534d618555f3d26e88dabe95f364f6287f83c4d0f71d29b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>BLAST search</topic><topic>BLATTELLA GERMANICA</topic><topic>Blattellidae - genetics</topic><topic>cDNA library</topic><topic>ESTs</topic><topic>Expressed Sequence Tags</topic><topic>Female</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>novel genes</topic><topic>Original</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Alignment</topic><toplevel>online_resources</toplevel><creatorcontrib>Chung, H.S. (Kosin University College of Medicine, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Yu, H.S. 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(Kosin University College of Medicine, Busan, Republic of Korea)</au><au>Kim, S.M. (Kosin University College of Medicine, Busan, Republic of Korea)</au><au>Kim, J.Y. (Kosin University College of Medicine, Busan, Republic of Korea)</au><au>Ock, M.S. (Kosin University College of Medicine, Busan, Republic of Korea), E-mail: sunnyock@kosin.ac.kr</au><au>Jeong, H.J. 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These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function.</abstract><cop>Korea (South)</cop><pub>대한기생충학열대의학회</pub><pmid>16340304</pmid><doi>10.3347/kjp.2005.43.4.149</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Korean journal of parasitology, 2005-12, Vol.43 (4), p.149-156 |
| issn | 0023-4001 1738-0006 |
| language | eng |
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| source | PubMed Central Free |
| subjects | Animals Base Sequence BLAST search BLATTELLA GERMANICA Blattellidae - genetics cDNA library ESTs Expressed Sequence Tags Female Male Molecular Sequence Data novel genes Original Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment |
| title | Expressed sequence tags analysis of Blattella germanica |
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