On the Function of Pentameric Phospholamban: Ion Channel or Storage Form?

Phospholamban (PLN) is an integral membrane protein that inhibits the sarcoplasmic reticulum Ca2+-ATPase, thereby regulating muscle contractility. We report a combined electrochemical and theoretical study demonstrating that the pentameric PLN does not possess channel activity for conducting chlorid...

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Bibliographic Details
Published in:Biophysical journal 2009-05, Vol.96 (10), p.L60-L62
Main Authors: Becucci, Lucia, Cembran, Alessandro, Karim, Christine B., Thomas, David D., Guidelli, Rolando, Gao, Jiali, Veglia, Gianluigi
Format: Article
Language:English
Subjects:
Adenosine
BASIC BIOLOGICAL SCIENCES
Biophysical Letter
Calcium
Calcium - metabolism
Calcium-Binding Proteins - chemistry
Calcium-Binding Proteins - metabolism
Cell Membrane - chemistry
Cell Membrane - metabolism
Chemical compounds
CHLORIDES
Chlorides - metabolism
CONFIGURATION
Environmental Molecular Sciences Laboratory
GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
Ion Channels - chemistry
Ion Channels - metabolism
Ions
Membranes
Models, Molecular
MUSCLES
Protein Multimerization
Protein Structure, Quaternary
PROTEINS
RELAXATION
SARCOPLASMIC RETICULUM
STORAGE
Studies
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Summary:Phospholamban (PLN) is an integral membrane protein that inhibits the sarcoplasmic reticulum Ca2+-ATPase, thereby regulating muscle contractility. We report a combined electrochemical and theoretical study demonstrating that the pentameric PLN does not possess channel activity for conducting chloride or calcium ions across the lipid membrane. This suggests that the pentameric configuration of PLN primarily serves as a storage form for the regulatory function of muscle relaxation by the PLN monomer.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2009.03.013