Protection of chickens against avian influenza with non-replicating adenovirus-vectored vaccine

Summary Protective immunity against avian influenza (AI) virus was elicited in chickens by single-dose vaccination with a replication competent adenovirus (RCA)-free human adenovirus (Ad) vector encoding an H7 AI hemagglutinin (AdChNY94.H7). Chickens vaccinated in ovo with an Ad vector encoding an A...

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Bibliographic Details
Published in:Vaccine 2008-05, Vol.26 (21), p.2640-2646
Main Authors: Toro, Haroldo, Tang, De-chu C, Suarez, David L, Zhang, Jianfeng, Shi, Zhongkai
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adenovirus
Allergy and Immunology
Animals
Antibodies, Viral - blood
Antigens
Applied microbiology
Avian influenza
Biological and medical sciences
Chickens
Dose-Response Relationship, Immunologic
Embryos
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
Genetic Vectors
Genomes
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Human adenovirus
Influenza in Birds - immunology
Influenza in Birds - prevention & control
Influenza Vaccines - genetics
Influenza Vaccines - immunology
Microbiology
Miscellaneous
Pandemics
Pharynx - virology
Poultry
Risk reduction
Vaccine
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Virology
Viruses
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Summary:Summary Protective immunity against avian influenza (AI) virus was elicited in chickens by single-dose vaccination with a replication competent adenovirus (RCA)-free human adenovirus (Ad) vector encoding an H7 AI hemagglutinin (AdChNY94.H7). Chickens vaccinated in ovo with an Ad vector encoding an AI H5 (AdTW68.H5) previously described, which were subsequently vaccinated intramuscularly with AdChNY94.H7 post-hatch, responded with robust antibody titers against both the H5 and H7 AI proteins. Antibody responses to Ad vector in ovo vaccination follow a dose–response kinetic. The use of a synthetic AI H5 gene codon optimized to match the chicken cell tRNA pool was more potent than the cognate H5 gene. The use of Ad-vectored vaccines to increase resistance of chicken populations against multiple AI strains could reduce the risk of an avian-originating influenza pandemic in humans.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.02.056