Loading…

Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease

The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represent...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteomics 2009-08, Vol.72 (6), p.907-917
Main Authors: Drake, Richard R., White, Krista Y., Fuller, Thomas W., Igwe, Elena, Clements, Mary Ann, Nyalwidhe, Julius O., Given, Robert W., Lance, Raymond S., Semmes, O. John
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033
cites cdi_FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033
container_end_page 917
container_issue 6
container_start_page 907
container_title Journal of proteomics
container_volume 72
creator Drake, Richard R.
White, Krista Y.
Fuller, Thomas W.
Igwe, Elena
Clements, Mary Ann
Nyalwidhe, Julius O.
Given, Robert W.
Lance, Raymond S.
Semmes, O. John
description The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represents the fluid being secreted by the prostate following a digital rectal prostate massage, which in turn can be collected in voided urine post-exam. This collection is not disruptive to a standard urological exam, and it can be repeatedly collected from men across all prostatic disease states. A direct EPS fluid can also be collected under anesthesia prior to prostatectomy. While multiple genetic assays for prostate cancer detection are being developed for the shed epithelial cell fraction of EPS urines, the remaining fluid that contains many prostate-derived proteins has been minimally characterized. Approaches to optimization and standardization of EPS collection consistent with current urological exam and surgical practices are described, and initial proteomic and glycomic evaluations of the of EPS fluid are summarized for prostate specific antigen and prostatic acid phosphatase. Continued characterization of the prostate specific protein components of EPS urine combined with optimization of clinical collection procedures should facilitate discovery of new biomarkers for prostate cancer.
doi_str_mv 10.1016/j.jprot.2009.01.007
format article
fullrecord <record><control><sourceid>elsevier_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2720443</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1874391909000037</els_id><sourcerecordid>S1874391909000037</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033</originalsourceid><addsrcrecordid>eNp9kd1KxDAQhYMo7rr6BN7kBVonTdpsLxRk8Q8WvNHrkCZTzdptStJdFHx4211RvBEGZmD4DnPmEHLOIGXAiotVuuqC79MMoEyBpQDygEzZXBaJlFwe7maR8JKVE3IS4wqgYLKUx2TCSpFLnvMp-Vw0rnVGN9T4pkHTO99S3Vo6SqNrx95h6B1G6muK713AGHG3j73unaERTcCRi1QPRaPfBIO09oFWzq91eMOwg38R6yLqiKfkqNZNxLPvPiPPtzdPi_tk-Xj3sLheJmY4s0-EscBqU85FziArUWptNIe6KjQiKxBzCcIKmVUcOTA25zW3ppA6F9bWwPmMXO11u021Rmuw7YNuVBfccN2H8tqpv5vWvaoXv1WZzECIUYDvBcxgIQasf1gGagxDrdQuDDWGoYCpIYyButxTOHjbOgwqGoetQevC8GllvfuX_wJN9Jfu</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Drake, Richard R. ; White, Krista Y. ; Fuller, Thomas W. ; Igwe, Elena ; Clements, Mary Ann ; Nyalwidhe, Julius O. ; Given, Robert W. ; Lance, Raymond S. ; Semmes, O. John</creator><creatorcontrib>Drake, Richard R. ; White, Krista Y. ; Fuller, Thomas W. ; Igwe, Elena ; Clements, Mary Ann ; Nyalwidhe, Julius O. ; Given, Robert W. ; Lance, Raymond S. ; Semmes, O. John</creatorcontrib><description>The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represents the fluid being secreted by the prostate following a digital rectal prostate massage, which in turn can be collected in voided urine post-exam. This collection is not disruptive to a standard urological exam, and it can be repeatedly collected from men across all prostatic disease states. A direct EPS fluid can also be collected under anesthesia prior to prostatectomy. While multiple genetic assays for prostate cancer detection are being developed for the shed epithelial cell fraction of EPS urines, the remaining fluid that contains many prostate-derived proteins has been minimally characterized. Approaches to optimization and standardization of EPS collection consistent with current urological exam and surgical practices are described, and initial proteomic and glycomic evaluations of the of EPS fluid are summarized for prostate specific antigen and prostatic acid phosphatase. Continued characterization of the prostate specific protein components of EPS urine combined with optimization of clinical collection procedures should facilitate discovery of new biomarkers for prostate cancer.</description><identifier>ISSN: 1874-3919</identifier><identifier>EISSN: 1876-7737</identifier><identifier>DOI: 10.1016/j.jprot.2009.01.007</identifier><identifier>PMID: 19457353</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Biomarker ; Prostate cancer ; Prostate specific antigen ; Prostatic acid phosphatase ; Proximal fluid</subject><ispartof>Journal of proteomics, 2009-08, Vol.72 (6), p.907-917</ispartof><rights>2009 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033</citedby><cites>FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Drake, Richard R.</creatorcontrib><creatorcontrib>White, Krista Y.</creatorcontrib><creatorcontrib>Fuller, Thomas W.</creatorcontrib><creatorcontrib>Igwe, Elena</creatorcontrib><creatorcontrib>Clements, Mary Ann</creatorcontrib><creatorcontrib>Nyalwidhe, Julius O.</creatorcontrib><creatorcontrib>Given, Robert W.</creatorcontrib><creatorcontrib>Lance, Raymond S.</creatorcontrib><creatorcontrib>Semmes, O. John</creatorcontrib><title>Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease</title><title>Journal of proteomics</title><description>The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represents the fluid being secreted by the prostate following a digital rectal prostate massage, which in turn can be collected in voided urine post-exam. This collection is not disruptive to a standard urological exam, and it can be repeatedly collected from men across all prostatic disease states. A direct EPS fluid can also be collected under anesthesia prior to prostatectomy. While multiple genetic assays for prostate cancer detection are being developed for the shed epithelial cell fraction of EPS urines, the remaining fluid that contains many prostate-derived proteins has been minimally characterized. Approaches to optimization and standardization of EPS collection consistent with current urological exam and surgical practices are described, and initial proteomic and glycomic evaluations of the of EPS fluid are summarized for prostate specific antigen and prostatic acid phosphatase. Continued characterization of the prostate specific protein components of EPS urine combined with optimization of clinical collection procedures should facilitate discovery of new biomarkers for prostate cancer.</description><subject>Biomarker</subject><subject>Prostate cancer</subject><subject>Prostate specific antigen</subject><subject>Prostatic acid phosphatase</subject><subject>Proximal fluid</subject><issn>1874-3919</issn><issn>1876-7737</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kd1KxDAQhYMo7rr6BN7kBVonTdpsLxRk8Q8WvNHrkCZTzdptStJdFHx4211RvBEGZmD4DnPmEHLOIGXAiotVuuqC79MMoEyBpQDygEzZXBaJlFwe7maR8JKVE3IS4wqgYLKUx2TCSpFLnvMp-Vw0rnVGN9T4pkHTO99S3Vo6SqNrx95h6B1G6muK713AGHG3j73unaERTcCRi1QPRaPfBIO09oFWzq91eMOwg38R6yLqiKfkqNZNxLPvPiPPtzdPi_tk-Xj3sLheJmY4s0-EscBqU85FziArUWptNIe6KjQiKxBzCcIKmVUcOTA25zW3ppA6F9bWwPmMXO11u021Rmuw7YNuVBfccN2H8tqpv5vWvaoXv1WZzECIUYDvBcxgIQasf1gGagxDrdQuDDWGoYCpIYyButxTOHjbOgwqGoetQevC8GllvfuX_wJN9Jfu</recordid><startdate>20090820</startdate><enddate>20090820</enddate><creator>Drake, Richard R.</creator><creator>White, Krista Y.</creator><creator>Fuller, Thomas W.</creator><creator>Igwe, Elena</creator><creator>Clements, Mary Ann</creator><creator>Nyalwidhe, Julius O.</creator><creator>Given, Robert W.</creator><creator>Lance, Raymond S.</creator><creator>Semmes, O. John</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20090820</creationdate><title>Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease</title><author>Drake, Richard R. ; White, Krista Y. ; Fuller, Thomas W. ; Igwe, Elena ; Clements, Mary Ann ; Nyalwidhe, Julius O. ; Given, Robert W. ; Lance, Raymond S. ; Semmes, O. John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biomarker</topic><topic>Prostate cancer</topic><topic>Prostate specific antigen</topic><topic>Prostatic acid phosphatase</topic><topic>Proximal fluid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Drake, Richard R.</creatorcontrib><creatorcontrib>White, Krista Y.</creatorcontrib><creatorcontrib>Fuller, Thomas W.</creatorcontrib><creatorcontrib>Igwe, Elena</creatorcontrib><creatorcontrib>Clements, Mary Ann</creatorcontrib><creatorcontrib>Nyalwidhe, Julius O.</creatorcontrib><creatorcontrib>Given, Robert W.</creatorcontrib><creatorcontrib>Lance, Raymond S.</creatorcontrib><creatorcontrib>Semmes, O. John</creatorcontrib><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Drake, Richard R.</au><au>White, Krista Y.</au><au>Fuller, Thomas W.</au><au>Igwe, Elena</au><au>Clements, Mary Ann</au><au>Nyalwidhe, Julius O.</au><au>Given, Robert W.</au><au>Lance, Raymond S.</au><au>Semmes, O. John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease</atitle><jtitle>Journal of proteomics</jtitle><date>2009-08-20</date><risdate>2009</risdate><volume>72</volume><issue>6</issue><spage>907</spage><epage>917</epage><pages>907-917</pages><issn>1874-3919</issn><eissn>1876-7737</eissn><abstract>The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represents the fluid being secreted by the prostate following a digital rectal prostate massage, which in turn can be collected in voided urine post-exam. This collection is not disruptive to a standard urological exam, and it can be repeatedly collected from men across all prostatic disease states. A direct EPS fluid can also be collected under anesthesia prior to prostatectomy. While multiple genetic assays for prostate cancer detection are being developed for the shed epithelial cell fraction of EPS urines, the remaining fluid that contains many prostate-derived proteins has been minimally characterized. Approaches to optimization and standardization of EPS collection consistent with current urological exam and surgical practices are described, and initial proteomic and glycomic evaluations of the of EPS fluid are summarized for prostate specific antigen and prostatic acid phosphatase. Continued characterization of the prostate specific protein components of EPS urine combined with optimization of clinical collection procedures should facilitate discovery of new biomarkers for prostate cancer.</abstract><pub>Elsevier B.V</pub><pmid>19457353</pmid><doi>10.1016/j.jprot.2009.01.007</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1874-3919
ispartof Journal of proteomics, 2009-08, Vol.72 (6), p.907-917
issn 1874-3919
1876-7737
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2720443
source ScienceDirect Freedom Collection 2022-2024
subjects Biomarker
Prostate cancer
Prostate specific antigen
Prostatic acid phosphatase
Proximal fluid
title Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T07%3A09%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-elsevier_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20collection%20and%20protein%20properties%20of%20expressed%20prostatic%20secretions%20as%20a%20source%20for%20biomarkers%20of%20prostatic%20disease&rft.jtitle=Journal%20of%20proteomics&rft.au=Drake,%20Richard%20R.&rft.date=2009-08-20&rft.volume=72&rft.issue=6&rft.spage=907&rft.epage=917&rft.pages=907-917&rft.issn=1874-3919&rft.eissn=1876-7737&rft_id=info:doi/10.1016/j.jprot.2009.01.007&rft_dat=%3Celsevier_pubme%3ES1874391909000037%3C/elsevier_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c457t-4cd01fc98451029e7aaca30fb6aee16ee5704d472b3e301183f3dc67a54ddf033%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/19457353&rfr_iscdi=true