Circulating Anti-Heat-Shock-Protein Antibodies in Normal Pregnancy and Preeclampsia

• Published in: Cell stress & chaperones 2009-09, Vol.14 (5), p.491-498
• Main Authors: Molvarec, Attila, Derzsy, Zoltán, Kocsis, Judit, Bőze, Tamás, Nagy, Bálint, Balogh, Krisztián, Makó, Veronika, Cervenak, László, Mézes, Miklós, Karádi, István, Prohászka, Zoltán, Rigó, János
• Format: Article
• Language: English
• Subjects: Adult; Antibodies; Antibodies - blood; Antibodies - immunology; Antigens; Biochemistry; Biomedical and Life Sciences; Biomedicine; Blood; Blood plasma; Body mass index; Cancer Research; Cell Biology; Chaperonin 60 - immunology; Female; Gestational age; Heat shock proteins; Heat-Shock Proteins - immunology; HSP70 Heat-Shock Proteins - immunology; Humans; Immunology; Neurosciences; Original Paper; Oxidative stress; Pre-Eclampsia - blood; Pre-Eclampsia - immunology; Preeclampsia; Pregnancy
• ISSN: 1355-8145; 1466-1268
• DOI: 10.1007/s12192-009-0102-4

It has been previously reported that circulating anti-heat-shock-protein (Hsp) antibody levels are elevated in cardiovascular disorders. The aim of the present study was to determine circulating antihuman Hsp60, antimycobacterial Hsp65, and antihuman Hsp70 antibody levels in healthy pregnant women and preeclamptic patients and to investigate their relationship to the clinical characteristics of the study subjects, as well as to the markers of inflammation (C-reactive protein (CRP)), endothelial activation (von Willebrand factor antigen), or endothelial injury (fibronectin), oxidative stress (malondialdehyde) and to serum Hsp70 levels. Ninety-three preeclamptic patients and 127 normotensive healthy pregnant women were involved in this case control study. Serum anti-Hsp60, anti-Hsp65, anti-Hsp70, and Hsp70 levels were measured with enzyme-linked immunosorbent assay (ELISA). Serum CRP levels were determined by an autoanalyzer using the manufacturer's kit. Plasma von Willebrand factor antigen levels were quantified by ELISA, while plasma fibronectin concentration by nephelometry. Plasma malondialdehyde levels were measured by the thiobarbituric-acid-based colorimetric assay. For statistical analyses, nonparametric methods were applied. Anti-Hsp60, anti-Hsp65, and anti-Hsp70 antibodies were detected in all of our serum samples. There were no significant differences in serum anti-Hsp60, anti-Hsp65, and anti-Hsp70 antibody levels between the control and preeclamptic groups. Serum levels of Hsp70 and CRP, as well as plasma levels of VWF antigen, fibronectin, and malondialdehyde, were significantly higher in preeclamptic patients than in normotensive healthy pregnant women. Serum anti-Hsp60 antibody levels showed significant correlations with serum anti-Hsp65 antibody levels both in the control and the preeclamptic groups (Spearman R=0.55 and 0.59; p