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Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness
We found neural crest stem cells (NCSCs) in the adult gut. Postnatal gut NCSCs were isolated by flow-cytometry and compared to fetal gut NCSCs. They self-renewed extensively in culture but less than fetal gut NCSCs. Postnatal gut NCSCs made neurons that expressed a variety of neurotransmitters but l...
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| Published in: | Neuron (Cambridge, Mass.) Mass.), 2002-08, Vol.35 (4), p.657-669 |
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| Main Authors: | , , , , , |
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| cites | cdi_FETCH-LOGICAL-c640t-347b79ab02b47b1fcd353a95e8bd2028cb485d33d225786c11c7a835faa198453 |
| container_end_page | 669 |
| container_issue | 4 |
| container_start_page | 657 |
| container_title | Neuron (Cambridge, Mass.) |
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| creator | Kruger, Genevieve M. Mosher, Jack T. Bixby, Suzanne Joseph, Nancy Iwashita, Toshihide Morrison, Sean J. |
| description | We found neural crest stem cells (NCSCs) in the adult gut. Postnatal gut NCSCs were isolated by flow-cytometry and compared to fetal gut NCSCs. They self-renewed extensively in culture but less than fetal gut NCSCs. Postnatal gut NCSCs made neurons that expressed a variety of neurotransmitters but lost the ability to make certain subtypes of neurons that are generated during fetal development. Postnatal gut NCSCs also differed in their responsiveness to lineage determination factors, affecting cell fate determination in vivo and possibly explaining their reduced neuronal subtype potential. These perinatal changes in gut NCSCs parallel perinatal changes in hematopoietic stem cells, suggesting that stem cells in different tissues undergo similar developmental transitions. The persistence of NCSCs in the adult PNS opens up new possibilities for regeneration after injury or disease. |
| doi_str_mv | 10.1016/S0896-6273(02)00827-9 |
| format | article |
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Postnatal gut NCSCs were isolated by flow-cytometry and compared to fetal gut NCSCs. They self-renewed extensively in culture but less than fetal gut NCSCs. Postnatal gut NCSCs made neurons that expressed a variety of neurotransmitters but lost the ability to make certain subtypes of neurons that are generated during fetal development. Postnatal gut NCSCs also differed in their responsiveness to lineage determination factors, affecting cell fate determination in vivo and possibly explaining their reduced neuronal subtype potential. These perinatal changes in gut NCSCs parallel perinatal changes in hematopoietic stem cells, suggesting that stem cells in different tissues undergo similar developmental transitions. 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The persistence of NCSCs in the adult PNS opens up new possibilities for regeneration after injury or disease.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Bone Morphogenetic Proteins - pharmacology</subject><subject>Catecholamines - biosynthesis</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage - drug effects</subject><subject>Cell Lineage - physiology</subject><subject>Cells, Cultured</subject><subject>Digestive System - cytology</subject><subject>Digestive System - embryology</subject><subject>Digestive System - innervation</subject><subject>Enteric Nervous System - cytology</subject><subject>Enteric Nervous System - embryology</subject><subject>Enteric Nervous System - metabolism</subject><subject>Fetus</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Flow Cytometry</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Proteins - pharmacology</subject><subject>Mitosis - drug effects</subject><subject>Mitosis - genetics</subject><subject>Nerve Growth Factors - metabolism</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Neural Crest - cytology</subject><subject>Neural Crest - embryology</subject><subject>Neural Crest - metabolism</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - metabolism</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor, Nerve Growth Factor - metabolism</subject><subject>Serotonin - biosynthesis</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkl1rFDEUhgdR7Fr9CUpAEAVHk8xMPm6UMtgqFC1dex0ymTO7KbPJmmRW-hv802a6S_246UVISJ7z5s2bUxTPCX5HMGHvl1hIVjLKq9eYvsFYUF7KB8WCYMnLmkj5sFjcIUfFkxivMSZ1I8nj4ohQImvB2KL49RWmoEfUBogJLRNsUAvjGNEFhGjzlnUorQGd9NOY0NmUUJfHleshrDxq19qtIM7QEsahvAQHP_X4Fs2q3mXd5dSlmy2gC5_AJTufadejU22SD-gS4ta7aHe5LsanxaNBjxGeHebj4ur00_f2c3n-7exLe3JeGlbjVFY177jUHaZdXpHB9FVTadmA6HqKqTBdLZq-qnpKGy6YIcRwLapm0JpIUTfVcfFhr7udug30JhvLEahtsBsdbpTXVv174uxarfxOUU5Fw1kWeHUQCP7HlINTGxtNjk078FNUPNuQhPB7QSIYZpJUGXz5H3jtp5ADzEyDK87q7DxTzZ4ywccYYLjzTLCau0LddoWav1xhqm67Qslc9-LvB_-pOrRBBj7uAcix7ywEFY0FZ6C3AUxSvbf3XPEbPBjIMg</recordid><startdate>20020815</startdate><enddate>20020815</enddate><creator>Kruger, Genevieve M.</creator><creator>Mosher, Jack T.</creator><creator>Bixby, Suzanne</creator><creator>Joseph, Nancy</creator><creator>Iwashita, Toshihide</creator><creator>Morrison, Sean J.</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020815</creationdate><title>Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness</title><author>Kruger, Genevieve M. ; Mosher, Jack T. ; Bixby, Suzanne ; Joseph, Nancy ; Iwashita, Toshihide ; Morrison, Sean J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c640t-347b79ab02b47b1fcd353a95e8bd2028cb485d33d225786c11c7a835faa198453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Bone Morphogenetic Proteins - metabolism</topic><topic>Bone Morphogenetic Proteins - pharmacology</topic><topic>Catecholamines - biosynthesis</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Lineage - drug effects</topic><topic>Cell Lineage - physiology</topic><topic>Cells, Cultured</topic><topic>Digestive System - cytology</topic><topic>Digestive System - embryology</topic><topic>Digestive System - innervation</topic><topic>Enteric Nervous System - cytology</topic><topic>Enteric Nervous System - embryology</topic><topic>Enteric Nervous System - metabolism</topic><topic>Fetus</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Flow Cytometry</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Proteins - pharmacology</topic><topic>Mitosis - drug effects</topic><topic>Mitosis - genetics</topic><topic>Nerve Growth Factors - metabolism</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Neural Crest - cytology</topic><topic>Neural Crest - embryology</topic><topic>Neural Crest - metabolism</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - metabolism</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor, Nerve Growth Factor - metabolism</topic><topic>Serotonin - biosynthesis</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kruger, Genevieve M.</creatorcontrib><creatorcontrib>Mosher, Jack T.</creatorcontrib><creatorcontrib>Bixby, Suzanne</creatorcontrib><creatorcontrib>Joseph, Nancy</creatorcontrib><creatorcontrib>Iwashita, Toshihide</creatorcontrib><creatorcontrib>Morrison, Sean J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kruger, Genevieve M.</au><au>Mosher, Jack T.</au><au>Bixby, Suzanne</au><au>Joseph, Nancy</au><au>Iwashita, Toshihide</au><au>Morrison, Sean J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>2002-08-15</date><risdate>2002</risdate><volume>35</volume><issue>4</issue><spage>657</spage><epage>669</epage><pages>657-669</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><abstract>We found neural crest stem cells (NCSCs) in the adult gut. Postnatal gut NCSCs were isolated by flow-cytometry and compared to fetal gut NCSCs. They self-renewed extensively in culture but less than fetal gut NCSCs. Postnatal gut NCSCs made neurons that expressed a variety of neurotransmitters but lost the ability to make certain subtypes of neurons that are generated during fetal development. Postnatal gut NCSCs also differed in their responsiveness to lineage determination factors, affecting cell fate determination in vivo and possibly explaining their reduced neuronal subtype potential. These perinatal changes in gut NCSCs parallel perinatal changes in hematopoietic stem cells, suggesting that stem cells in different tissues undergo similar developmental transitions. 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| ispartof | Neuron (Cambridge, Mass.), 2002-08, Vol.35 (4), p.657-669 |
| issn | 0896-6273 1097-4199 |
| language | eng |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
| subjects | Aging - metabolism Animals Bone Morphogenetic Proteins - metabolism Bone Morphogenetic Proteins - pharmacology Catecholamines - biosynthesis Cell Differentiation - drug effects Cell Differentiation - physiology Cell Lineage - drug effects Cell Lineage - physiology Cells, Cultured Digestive System - cytology Digestive System - embryology Digestive System - innervation Enteric Nervous System - cytology Enteric Nervous System - embryology Enteric Nervous System - metabolism Fetus Fibroblasts - cytology Fibroblasts - metabolism Flow Cytometry Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Developmental - physiology Intracellular Signaling Peptides and Proteins Membrane Proteins - metabolism Membrane Proteins - pharmacology Mitosis - drug effects Mitosis - genetics Nerve Growth Factors - metabolism Nerve Growth Factors - pharmacology Neural Crest - cytology Neural Crest - embryology Neural Crest - metabolism Neuroglia - cytology Neuroglia - metabolism Neurons Neurons - cytology Neurons - metabolism Rats Rats, Sprague-Dawley Receptor, Nerve Growth Factor - metabolism Serotonin - biosynthesis Stem cells Stem Cells - cytology Stem Cells - metabolism |
| title | Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness |
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