Pericentromeric sister chromatid cohesion promotes kinetochore biorientation

Accurate chromosome segregation depends on sister kinetochores making bioriented attachments to microtubules from opposite poles. An essential regulator of biorientation is the Ipl1/Aurora B protein kinase that destabilizes improper microtubule-kinetochore attachments. To identify additional biorien...

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Bibliographic Details
Published in:Molecular biology of the cell 2009-09, Vol.20 (17), p.3818-3827
Main Authors: Ng, Tessie M, Waples, William G, Lavoie, Brigitte D, Biggins, Sue
Format: Article
Language:English
Subjects:
Aurora Kinases
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Chromatids - metabolism
Chromosome Segregation
Epistasis, Genetic
Intracellular Signaling Peptides and Proteins - genetics
Intracellular Signaling Peptides and Proteins - metabolism
Kinetochores - metabolism
Lac Repressors - genetics
Lac Repressors - metabolism
Microtubules - metabolism
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Spindle Apparatus - metabolism
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Summary:Accurate chromosome segregation depends on sister kinetochores making bioriented attachments to microtubules from opposite poles. An essential regulator of biorientation is the Ipl1/Aurora B protein kinase that destabilizes improper microtubule-kinetochore attachments. To identify additional biorientation pathways, we performed a systematic genetic analysis between the ipl1-321 allele and all nonessential budding yeast genes. One of the mutants, mcm21Delta, precociously separates pericentromeres and this is associated with a defect in the binding of the Scc2 cohesin-loading factor at the centromere. Strikingly, Mcm21 becomes essential for biorientation when Ipl1 function is reduced, and this appears to be related to its role in pericentromeric cohesion. When pericentromeres are artificially tethered, Mcm21 is no longer needed for biorientation despite decreased Ipl1 activity. Taken together, these data reveal a specific role for pericentromeric linkage in ensuring kinetochore biorientation.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e09-04-0330