Protein factors in pre-mRNA 3'-end processing

Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3'-end. Processing at the 3'-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplic...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2008-04, Vol.65 (7-8), p.1099-1122
Main Authors: Mandel, C. R, Bai, Y, Tong, L
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
cleavage and polyadenylation specificity factor
cleavage stimulation factor
DNA-directed RNA polymerase
endoribonuclease
Enzymes
Eukaryotes
Humans
Life Sciences
Mammals
Mammals - metabolism
poly(A) polymerase
Protein complex
Proteins
Regulatory Sequences, Ribonucleic Acid - genetics
Review
Ribonucleic acid
RNA
RNA 3' End Processing
RNA Precursors - metabolism
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - metabolism
Saccharomyces cerevisiae - metabolism
Yeasts
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Summary:Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3'-end. Processing at the 3'-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity of the processing reactions, more than 14 proteins have been identified for the mammalian complex, and more than 20 proteins have been identified for the yeast complex. The 3'-end processing machinery also has important roles in transcription and splicing. The mammalian machinery contains several sub-complexes, including cleavage and polyadenylation specificity factor, cleavage stimulation factor, cleavage factor I, and cleavage factor II. Additional protein factors include poly(A) polymerase, poly(A)-binding protein, symplekin, and the C-terminal domain of RNA polymerase II largest subunit. The yeast machinery includes cleavage factor IA, cleavage factor IB, and cleavage and polyadenylation factor.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-007-7474-3