Two-Photon Luminescence Imaging of Cancer Cells Using Molecularly Targeted Gold Nanorods

We demonstrate the use of gold nanorods as bright contrast agents for two-photon luminescence (TPL) imaging of cancer cells in a three-dimensional tissue phantom down to 75 μm deep. The TPL intensity from gold-nanorod-labeled cancer cells is 3 orders of magnitude brighter than the two-photon autoflu...

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Bibliographic Details
Published in:Nano letters 2007-04, Vol.7 (4), p.941-945
Main Authors: Durr, Nicholas J, Larson, Timothy, Smith, Danielle K, Korgel, Brian A, Sokolov, Konstantin, Ben-Yakar, Adela
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinoma, Squamous Cell - pathology
Cell Line, Tumor
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Contrast Media - chemistry
Cross-disciplinary physics: materials science
rheology
Drug Delivery Systems - methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Gold Colloid - chemistry
Humans
Image Enhancement - methods
Luminescent Measurements - methods
Materials science
Materials Testing
Microscopy, Fluorescence, Multiphoton - methods
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Nanotechnology - methods
Nanotubes - chemistry
Nanotubes - ultrastructure
Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation
Optical properties of low-dimensional, mesoscopic, and nanoscale materials and structures
Particle Size
Physics
Tissues, organs and organisms biophysics
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Summary:We demonstrate the use of gold nanorods as bright contrast agents for two-photon luminescence (TPL) imaging of cancer cells in a three-dimensional tissue phantom down to 75 μm deep. The TPL intensity from gold-nanorod-labeled cancer cells is 3 orders of magnitude brighter than the two-photon autofluorescence (TPAF) emission intensity from unlabeled cancer cells at 760 nm excitation light. Their strong signal, resistance to photobleaching, chemical stability, ease of synthesis, simplicity of conjugation chemistry, and biocompatibility make gold nanorods an attractive contrast agent for two-photon imaging of epithelial cancer.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl062962v