Improved base calling for the Illumina Genome Analyzer using machine learning strategies

The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with resp...

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Bibliographic Details
Published in:Genome biology 2009-01, Vol.10 (8), p.R83-R83, Article R83
Main Authors: Kircher, Martin, Stenzel, Udo, Kelso, Janet
Format: Article
Language:English
Subjects:
Algorithms
Artificial Intelligence
chemistry
Genome
Humans
molecular genetics
Sequence Analysis, DNA - methods
Software
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Summary:The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with respect to chemistry and technology than other publicly available packages. Ibis is freely available under the GPL from http://bioinf.eva.mpg.de/Ibis/.
ISSN:1474-760X
1465-6906
1474-760X
1465-6914
DOI:10.1186/gb-2009-10-8-r83