Determination of glycosylation sites and site-specific heterogeneity in glycoproteins

Glycosylation is one of the most common post-translational modifications (PTMs) of proteins. At least 50% of human proteins are glycosylated with some estimates being as high as 70%. Glycoprotein analysis requires determining both the sites of glycosylation as well as the glycan structures associate...

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Bibliographic Details
Published in:Current opinion in chemical biology 2009-10, Vol.13 (4), p.421-426
Main Authors: An, Hyun Joo, Froehlich, John W, Lebrilla, Carlito B
Format: Article
Language:English
Subjects:
Animals
Glycoproteins - analysis
Glycosylation
Humans
Mass Spectrometry - methods
Polysaccharides - analysis
Protein Processing, Post-Translational
Proteomics - methods
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Summary:Glycosylation is one of the most common post-translational modifications (PTMs) of proteins. At least 50% of human proteins are glycosylated with some estimates being as high as 70%. Glycoprotein analysis requires determining both the sites of glycosylation as well as the glycan structures associated with each site. Recent advances have led to the development of new analytical methods that employ mass spectrometry extensively making it possible to obtain the glycosylation site and the site microheterogeneity. These tools will be important for the eventual development of glycoproteomics.
ISSN:1367-5931
1879-0402
DOI:10.1016/j.cbpa.2009.07.022