Mmp-20 and Klk4 Cleavage Site Preferences for Amelogenin Sequences

Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them to digest the tyrosine-rich amelogenin polypep...

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Bibliographic Details
Published in:Journal of dental research 2009-09, Vol.88 (9), p.823-828
Main Authors: Nagano, T., Kakegawa, A., Yamakoshi, Y., Tsuchiya, S., Hu, J.C.-C., Gomi, K., Arai, T., Bartlett, J.D., Simmer, J.P.
Format: Article
Language:English
Subjects:
Alanine - metabolism
Amelogenesis - physiology
Amelogenin - metabolism
Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid
Chromatography, Liquid
Dental Enamel - secretion
Dental Enamel Proteins - analysis
Dentistry
Electrophoresis, Polyacrylamide Gel
Histidine - metabolism
Kallikreins - metabolism
Matrix Metalloproteinase 20 - metabolism
Proline - metabolism
Research Reports
Serine - metabolism
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Swine
Tandem Mass Spectrometry
Tryptophan - metabolism
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Summary:Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them to digest the tyrosine-rich amelogenin polypeptide (TRAP), the leucine-rich amelogenin protein (LRAP), and 5 fluorescence peptides. We characterized the digestion products by LC-MSMS, SDS-PAGE, and C18 RP-HPLC monitored with fluorescence and UV detectors. Mmp-20 cleaves amelogenin sequences after Pro162, Ser148, His62, Ala63, and Trp45. These cleavages generate all of the major cleavage products that accumulate in porcine secretory-stage enamel: the 23-kDa, 20-kDa, 13-kDa, 11-kDa, and 6-kDa (TRAP) amelogenins. Mmp-20 cleaves LRAP after Pro45 and Pro40, producing the two LRAP products previously identified in tooth extracts. Among these key cleavage sites, Klk4 was able to cleave only after His62. We propose that Mmp-20 alone processes amelogenin during the secretory stage.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034509342694