Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cel...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2009-05, Vol.324 (5928), p.797-801
Main Authors: Yu, Junying, Hu, Kejin, Smuga-Otto, Kim, Tian, Shulan, Stewart, Ron, Slukvin, Igor I., Thomson, James A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell lines
Cell physiology
Cell Shape
Cellular biology
Cellular Reprogramming
Clone Cells
Embryonic stem cells
Embryonic Stem Cells - cytology
Epstein-Barr Virus Nuclear Antigens - genetics
Fibroblasts
Foreskin
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Genetic engineering
Genetic Vectors
Genomics
Humans
Induced pluripotent stem cells
Liver cells
Molecular and cellular biology
Plasmids
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Pluripotent Stem Cells - transplantation
Somatic cells
Stem cells
Transcription Factors - genetics
Transfection
Transgenes
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Summary:Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cells in both research and clinical applications. We describe the derivation of human iPS cells with the use of nonintegrating episomal vectors. After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem (ES) cells in proliferative and developmental potential. These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1172482