Proteomic analysis of circulating monocytes in Chinese premenopausal females with extremely discordant bone mineral density

Osteoporosis (OP) is a major public health problem, mainly characterized by low bone mineral density (BMD). Circulating monocytes (CMCs) may serve as progenitors of osteoclasts and produce a wide variety of factors important to bone metabolism. However, the specific action mechanism of CMCs in the p...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2008-10, Vol.8 (20), p.4259-4272
Main Authors: Deng, Fei-Yan, Liu, Yao-Zhong, Li, Li-Ming, Jiang, Chen, Wu, Shan, Chen, Yuan, Jiang, Hui, Yang, Fang, Xiong, Ji-Xian, Xiao, Peng, Xiao, Su-Mei, Tan, Li-Jun, Sun, Xiao, Zhu, Xue-Zhen, Liu, Man-Yuan, Lei, Shu-Feng, Chen, Xiang-Ding, Xie, Jing-Yun, Xiao, Gary G, Liang, Song-Ping, Deng, Hong-Wen
Format: Article
Language:English
Subjects:
Adult
Analytical, structural and metabolic biochemistry
Asian People
Biological and medical sciences
Bone Density - physiology
Bone mineral density
China
Circulating monocyte
Female
Fundamental and applied biological sciences. Psychology
Gelsolin - metabolism
Gene Expression Profiling
Glutathione Peroxidase - metabolism
Glutathione Peroxidase GPX1
Humans
Miscellaneous
Monocytes - metabolism
Osteoclastogenesis
Osteoporosis - etiology
Premenopause - physiology
Procollagen-Proline Dioxygenase - metabolism
Protein
Protein Disulfide-Isomerases - metabolism
Proteins
Superoxide Dismutase - metabolism
Transcription Factors - metabolism
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Summary:Osteoporosis (OP) is a major public health problem, mainly characterized by low bone mineral density (BMD). Circulating monocytes (CMCs) may serve as progenitors of osteoclasts and produce a wide variety of factors important to bone metabolism. However, the specific action mechanism of CMCs in the pathogenesis of OP is far from clear. We performed a comparative protein expression profiling study of CMCs in Chinese premenopausal females with extremely discordant BMD, identified a total of 38 differentially expressed proteins, and confirmed with Western blotting five proteins: ras suppressor protein1 (RSU1), gelsolin (GSN), manganese-containing superoxide dismutase (SOD2), glutathione peroxidase 1(GPX1), and prolyl 4-hydroxylase β subunit (P4HB). These proteins might affect CMCs' trans-endothelium, differentiation, and/or downstream osteoclast functions, thus contribute to differential osteoclastogenesis and finally lead to BMD variation. The findings promote our understanding of the role of CMCs in BMD determination, and provide an insight into the pathogenesis of human OP.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200700480