Salivary analysis of oral cancer biomarkers

• Published in: British journal of cancer 2009-10, Vol.101 (7), p.1194-1198
• Main Authors: Shpitzer, T, Hamzany, Y, Bahar, G, Feinmesser, R, Savulescu, D, Borovoi, I, Gavish, M, Nagler, R M
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Antibodies; Biochemistry; Biological and medical sciences; Biomarkers; Biomarkers, Tumor - analysis; Biomedical and Life Sciences; Biomedicine; Cancer Research; Carcinoma, Squamous Cell - diagnosis; Cyclin D1 - analysis; Dehydrogenases; DNA Glycosylases - analysis; Drug Resistance; Epidemiology; Female; Humans; Ki-67 Antigen - analysis; Male; Matrix Metalloproteinase 9 - analysis; Medical research; Medical sciences; Middle Aged; Molecular Diagnostics; Molecular Medicine; Neoplasm Staging; Oncology; Oral cancer; Otolaryngology; Otorhinolaryngology. Stomatology; Saliva - chemistry; Sensitivity and Specificity; Tongue; Tongue Neoplasms - diagnosis; Tongue Neoplasms - pathology; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1038/sj.bjc.6605290

Background: Oral cancer is a common and lethal malignancy. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing. Methods: We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress, DNA repair, carcinogenesis, metastasis and cellular proliferation and death. Results: Five markers increased in cancer patients by 39–246%: carbonyls, lactate dehydrogenase, metalloproteinase-9 (MMP-9), Ki67 and Cyclin D1 (CycD1) ( P ⩽0.01). Three markers decreased by 16–29%: 8-oxoguanine DNA glycosylase, phosphorylated-Src and mammary serine protease inhibitor (Maspin) ( P ⩽0.01). Increase in salivary carbonyls was profound (by 246%, P =0.012); alterations in CycD1 (87% increase, P =0.000006) and Maspin (29% decrease, P =0.007) were especially significant. Sensitivity values of these eight analysed markers ranged from 58% to 100%; specificity values ranged from 42% to 100%. Both values were especially high for the CycD1 and Maspin markers, 100% for each value of each marker. These were also high for carbonyls, 90% and 80%, respectively, and for MMP-9, 100% and 79%, respectively. Conclusion: The significance of each salivary alteration is discussed. As all alterations correlated with each other, they may belong to a single carcinogenetic network. Cancer-related changes in salivary tumour markers may be used as a diagnostic tool for diagnosis, prognosis and post-operative monitoring.