Identification of recombinant human papillomavirus type 16 variants

Abstract Intratypic diversity of human papillomavirus (HPV) genome is generally characterized by point mutation, insertion, and/or deletion. Using PCR-based cloning and sequencing, we detected concurrent infection with 8 HPV16 variants in a woman enrolled in the ASCUS-LSIL Triage Study. The European...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2009-11, Vol.394 (1), p.8-11
Main Authors: Jiang, Mingjun, Xi, Long Fu, Edelstein, Zoe R, Galloway, Denise A, Olsem, Gary J, Lin, William Chun-Che, Kiviat, Nancy B
Format: Article
Language:English
Subjects:
DNA, Viral - genetics
Female
Human papillomavirus
Human papillomavirus 16
Human papillomavirus 16 - genetics
Human papillomavirus 16 - isolation & purification
Humans
Infectious Disease
Papillomavirus Infections - virology
Polymerase Chain Reaction
Polymorphism, Genetic
Recombination
Recombination, Genetic
Sequence Analysis, DNA
Variant
Young Adult
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Summary:Abstract Intratypic diversity of human papillomavirus (HPV) genome is generally characterized by point mutation, insertion, and/or deletion. Using PCR-based cloning and sequencing, we detected concurrent infection with 8 HPV16 variants in a woman enrolled in the ASCUS-LSIL Triage Study. The European variant was the major variant; each of the 7 minor variants had partial DNA sequences identical to the European variant and another part identical to the African 2 variant. At a follow-up visit, only an HPV16 African 2 variant was detected. Results from the present study suggest presence of intratypic recombination of HPV genome in natural infection.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2009.08.040