Determinants of DNA yield and purity collected with buccal cell samples

Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1...

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Bibliographic Details
Published in:European journal of epidemiology 2009, Vol.24 (11), p.677-682
Main Authors: van Wieren-de Wijer, D. B. M. A, Maitland-van der Zee, A. H, de Boer, A, Belitser, S. V, Kroon, A. A, de Leeuw, P. W, Schiffers, P, Janssen, R. G. J. H, van Duijn, C. M, Stricker, B. H. C. H, Klungel, O. H
Format: Article
Language:English
Subjects:
Age Factors
Aged
Antihypertensive Agents - adverse effects
Antihypertensives
Average total cost
Biological and medical sciences
Blood specimen collection
Cardiology
Case-Control Studies
Cells
Deoxyribonucleic acid
Diuretics
DNA
DNA - isolation & purification
Epidemiology
Female
General aspects
Genomics
Humans
Infectious Diseases
Male
Medical sciences
Medicine
Medicine & Public Health
Men
Methods
Middle Aged
Miscellaneous
Mouth Mucosa - cytology
Mouth Mucosa - drug effects
Mouthwashes
Oncology
Public Health
Public health. Hygiene
Public health. Hygiene-occupational medicine
Sex Factors
Specimen Handling
Statistical median
Xerostomia
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Summary:Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 μg (median 54.3 μg; mean 82.2 μg ± SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 μg) than those from women (median 44.2 μg). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics.
ISSN:0393-2990
1573-7284
DOI:10.1007/s10654-009-9388-x