Rapid SYBR Green I and Modified Probe Real-Time Reverse Transcription-PCR Assays Identify Influenza H1N1 Viruses and Distinguish between Pandemic and Seasonal Strains

A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-11, Vol.47 (11), p.3714-3716
Main Authors: Yang, Ji-Rong, Lo, Je, Liu, Jian-Liang, Lin, Chao-Hua, Ho, Yu-Lin, Chen, Chun-Jung, Wu, Ho-Sheng, Liu, Ming-Tsan
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Humans
Influenza A Virus, H1N1 Subtype - classification
Influenza A Virus, H1N1 Subtype - genetics
Influenza A Virus, H1N1 Subtype - isolation & purification
Influenza, Human - virology
Microbiology
Miscellaneous
Oligonucleotide Probes - genetics
Organic Chemicals - metabolism
Reverse Transcriptase Polymerase Chain Reaction - methods
Sensitivity and Specificity
Staining and Labeling - methods
Time Factors
Viral Proteins - genetics
Virology
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Summary:A rapid SYBR green I real-time reverse transcription-PCR (RT-PCR) assay was developed to identify pandemic influenza H1N1 virus from clinical specimens in less than 1 h. Probe real-time RT-PCR influenza A/B, H1/H3, and swNP/swHA assays were modified into the same PCR program, which allows for rapid and simultaneous typing and subtyping of influenza viruses.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01646-09