A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film

Titanium dioxide （TiO2） thin film was deposited on the surface of the light addressable potentiometric sensor （LAPS） to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet （UV） treatment on the silanization level of TiO2 thin film by 3-amin...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2009-11, Vol.10 (11), p.860-866
Main Authors: Zong, Xiao-lin, Wu, Chun-sheng, Wu, Xiao-ling, Lu, Yun-feng, Wang, Ping
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Biosensing Techniques
Biotechnology
Deoxyribonucleic acid
DNA
DNA生物传感器
Equipment Design
Fluorescein - chemistry
Fluorescein-5-isothiocyanate - chemistry
Genetic testing
Hydrolysis
Light
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis - instrumentation
Oxygen - chemistry
Potentiometry - instrumentation
Potentiometry - methods
Propylamines
Silanes - chemistry
Thin films
Titanium - chemistry
Ultraviolet Rays
二氧化钛薄膜
光寻址电位传感器
异硫氰酸荧光素
紫外线照射
薄膜传感器
表面涂层材料
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Summary:Titanium dioxide （TiO2） thin film was deposited on the surface of the light addressable potentiometric sensor （LAPS） to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet （UV） treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane （APTS）, fluorescein isothiocyanate （FITC） was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that, with UV irradiation, the silani- zation level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and effi- ciency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B0920090