A systems biology approach to study the phagosomal proteome modulated by mycobacterial infections

Systems biology and proteomics have recently contributed significantly to the insight into the biogenesis and immunity-related functions of the phagosome. To gain insight into the modulation of the phagosomal proteome by the wild-type Mycobacterium tuberculosis H37Rv reference strain, an attenuated...

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Bibliographic Details
Published in:International journal of clinical and experimental medicine 2009-09, Vol.2 (3), p.233-247
Main Authors: Rao, Prahlad K, Singh, Christoher R, Jagannath, Chinnaswamy, Li, Qingbo
Format: Article
Language:English
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Summary:Systems biology and proteomics have recently contributed significantly to the insight into the biogenesis and immunity-related functions of the phagosome. To gain insight into the modulation of the phagosomal proteome by the wild-type Mycobacterium tuberculosis H37Rv reference strain, an attenuated mutant of the H37Rv strain, and the BCG Pasteur vaccine strain, we employed the nano-liquid chromatography/LTQ-FTMS based proteomics approach and a systems biology approach to analyze the bacillus-containing phagosomes purified from the bone-marrow-derived BMA3.A3 macrophages infected with the three different mycobacterial strains. We identified 322 proteins at a false-discovery rate of 2%. These proteins were quantified with a label-free proteomics method. All but one of these proteins is mouse proteins. The gene ontology analysis of these mouse proteins suggests that lysosomal proteins represented
ISSN:1940-5901